摘要
应用 PAGE 法对多房棘球绦虫的泡球蚴囊液、全囊、原头蚴及囊壁组织的乳酸脱氢酶(LDH)、酯酶(EST)和酸性磷酸酶(AP)的同工酶进行了分析.结果 LDH 同工酶仅囊液与全囊各出现4条酶带.EST 同工酶全囊、囊液、原头蚴和囊壁分别出现14、17、3和13条酶带;而 AP 同工酶4种样品的酶带则分别是2、4、1和1条.3种同工酶的酶带数均以囊液样本为多且染色深,提示囊液可能是泡球蚴物质代谢的一个重要场所.
This paper reports the studies on isoenzyms of lactic dehydrogenase (LDH),esterase (FAST) and acid phosphatsse(AP) in cyst fluid,hydatid cysts,protoscoleces and cyst walls of alveolar hydatid (metacestoda of Echnococcus multilocularis)by PAGE analyses.In LDH isoenzyme analysis only 4 enzymatic bands revealed in each specimen of cyst fluid and hy- datid cyst.In EST isoenzyme analysis 14,17,3 and 13 enzymaic bands were found in speci- mens of hydatid cysts,cyst fluid,protoscoleces and cyst walls respectively;while enzymatic bands of AP were 4,2,1 and 1 correspondingly in four specimens.The result of tests showed that in specimens of cyst fluid the number of enzymatic bands in three isoenzymes were more than that in other specimens and they were stained more strongly,so cyst fluid might play an improtsnt role in the metablism of alveolar hydatid.
