摘要
本文报道感染日本血吸虫兔及血吸虫病严重肝纤维化(晚血)患者 PBMCs 诱生 TNF-α能力及其血清水平观察结果。TNF-α用 L929细胞杀伤法测定。兔感染后8周时血清 TNF-α水平到达高峰(23.14±6.10U/ml),其后明显下降,28周仍高于正常兔,这一种动态变化类似于肝胶原合成的变化。经杀虫和杀虫加抗肝纤维化治疗后,TNF-α诱生能力与血清水平均明显下降,与肝纤维化程度、肝胶原量、透明质酸等指标一致。晚血患者亦获类似结果。提示 TNF-α可能是参与血吸虫病肝纤维化形成的调节因子之一。
Dynamic observations on the induced production of serum TNF-α and PBMCS-deri-ved TNF-α in rabbits infected with Schistosoma japonicum and in patients with hepatiefibrosis of schistosomaisis japonica(late cases)were reported.L929 cell cytotoxicityassay was used to determine the TNF-α activity.Peak levels of TNF-α(23.14±6.10U/ml)in rabbits were found at the 8th week then dropped at the 28th week after in-fection,but still higher than that of uninfected rabbits.Alteration of TNF-α level iinfected rabbits was similar to that of collagen synthesis.After schistosomicidand/or antifibrotic therapy,TNF-α induction ability and serum TNF-α level took asignificant drop correlated with severity of hepatic fibrosis and parameters such ashepatic collagen content,hyaluronic acid,etc.In late cases,serum TNF-a level wasmarkedly elevated and the TNF-α induction ability was significantly lowered.SerumTNF-α level was remarkably decreased after antifibrotic therapy.It suggests that theTNF-α is one of the important cytokines participated in the regulation of hepatic fib-rosis formation.
出处
《上海医学》
CAS
CSCD
北大核心
1993年第4期212-215,共4页
Shanghai Medical Journal
