摘要
用RT PCR方法对分离的TN野毒株H基因进行了扩增 ,并将其克隆到pGEM T载体上 ,进行核苷酸序列测定。结果TN野毒株H基因ORF全长 1 ,81 5bp ,编码 60 4个氨基酸。与GenBank中己报道的 7个CDV毒株相比 ,H基因核苷酸序列的同源性在 92 %～ 99%之间 ,推导的H蛋白氨基酸序列的同源性在 91 %～ 99%之间。TN株H蛋白潜在的N 联糖基化位点为 8个 ,而Onderstepoort弱毒株H蛋白为 4个 ;其中N端第 1 9～ 2 1、 30 9～ 31 1、 5 84～ 5 86位氨基酸所形成的 3个潜在的糖基化位点是野毒株拥有而疫苗株所没有的。预测的TN株和Onderstepoort株H蛋白的疏水性及抗原表位存在一定差异。研究结果提示TN野毒株H蛋白免疫原性可能与弱毒株有较大差别 。
H gene of wild strain TN was amplified by RT-PCR and cloned into pGEM-T. The correct positive recombinant was used for sequencing. The open reading frame of H gene of CDV TN was 1815 bp and encoded 604 amino acids. The homology of nucleic acid and amino acid among the 8 strains of CDV reported by GenBank database ranged from 92 to 99 percent and from 91 to 99 percent, respectively. There are obviously different in potential N-glycosylation sites. The strain TN owned 8 potential N-glycosylation sites but 4 potential sites for Onderstepoort strain. Three potential N-glycosylation sites which located in the position of 19～21、309～311, 584～586 amino acids were unique to yield isolates. Moreover, there were some differences in the hydrophobicity and antigenic index between two strains, which may be one of important reasons for immune failure of CD.
出处
《微生物学通报》
CAS
CSCD
北大核心
2004年第6期6-10,共5页
Microbiology China
关键词
犬瘟热病毒
TN野毒株
血凝蛋白基因
序列分析
Canine distemper virus, TN strain, Haemagglutinin gene, Sequence analysis
