摘要
采用溶液培养试验,并结合大田试验,研究和探讨了耐低磷水稻基因型的筛选指标.结果表明,溶液培养试验中,在正常供磷和低磷胁迫条件下,在所有调查性状中水稻单株干重都具有较大的基因型间变异(CV分别为2173%和1954%).在所有调查性状的相对值中,相对单株干重(低磷胁迫/正常供磷)也具有较大的基因型间变异(CV为1960%);相关分析表明,相对单株干重与相对根干重、相对株高、相对单株吸磷量、相对地上部磷积累、相对磷利用效率和相对植株磷浓度均呈极显著正相关(P<001).因此,水稻相对单株干重可以作为苗期筛选水稻耐低磷基因型的一个筛选指标.溶液培养试验中水稻的相对单株干重和大田试验中水稻的相对稻谷产量(不施磷/施磷)没有显著相关性,因此溶液培养试验的相对单株干重不能作为评价大田试验中水稻耐低磷能力的指标.低磷营养液培养的水稻体内磷利用效率与缺磷土壤生长的水稻体内磷利用效率呈极显著正相关.因此,直接以低磷营养液培养水稻苗期体内磷利用效率作为筛选指标,然后进行大田试验验证,是一条筛选水稻磷高效利用基因型的有效途径.
Screening and breeding low phosphorous(P)tolerant rice genotype is one of the effective alternatives to alleviate the scarcity of mineral P resource and to increase the utilization efficiency of P fertilizer.Selecting a simple and scientific screening index plays an important role in the screening and breeding program.In this study,a hydroponic culture experiment and a field trial were carried out to approach the screening index for low P tolerant rice genotypes.The results showed that among all the rice characters studied,total dry weight demonstrated a significant genotypic variation at both normal and low P supply(the CV was 21.73% and 19.54%,respectively).Relative total dry weight(low P supply/normal P supply)also demonstrated significant genotypic variation(CV was 19.60%),which was significantly correlated to relative root dry weight,relative plant height,relative total P uptake,relative shoot P accumulation,relative P utilization efficiency and relative P concentration(P<0.01).Therefore,the relative total dry weight of rice would be an important evaluation or screening index for the low P tolerance ability of rice at its seedling stage.The relative total dry weight of rice in hydroponic culture system was not correlated to the relative grain yield(minus-P/plus-P)in field trial,which meant that it could not be used as an index to evaluate the low P tolerance ability of rice.The internal P utilization efficiency of rice grown in low P solution was significantly correlated to that of rice grown in P-deficient soil(r=0.798~** ,n=8).Therefore,screening rice genotypes by using internal P utilization efficiency of rice grown in low P solution at seedling stage as screening index and subsequently testing in field trial would probably be an effective alternative to screen rice genotype with high P utilization efficiency.
出处
《应用生态学报》
CAS
CSCD
北大核心
2005年第1期119-124,共6页
Chinese Journal of Applied Ecology
基金
国家"863"计划资助项目(2001AA2410251)
关键词
水稻
耐低磷
基因型
筛选指标
评价指标
Oryza sativa, Low P tolerance, Genotype, Screening index, Evaluation index.
