摘要
研究了盐酸胍变性烟草多酚氧化酶后的复性。结果表明,在没有外加添加剂存在的条件下,最优的复性条件为:盐酸胍浓度为15mol/L;适宜的酶浓度为15×10-4g/mL;变性时间短(30min);复性温度低(4℃),此时,复性收率最高达到6336%。10mol/L硫酸铵具有一定的促进烟草多酚氧化酶复性的作用,与此相比,100μmol/L硫酸铜促进烟草多酚氧化酶复性的效果较大,硫酸铜和硫酸铵存在协同作用,联合使用可获得最高的烟草多酚氧化酶复性收率;β环糊精对变性烟草多酚氧化酶的复性具有一定辅助作用,溴化十六烷基三甲基铵(CTAB)可增强β环糊精辅助变性蛋白质复性的作用。
The renaturation of polyphenol oxidase from Tobacco denatured in 6 0 mol/L guanidine hydrochloride(GdnHCl) was investigated. In the absence of additives(s), the optimal renaturation conditions, with 63 36% of activity recovered, are as follows:1 5 mol/L GdnHCl, denaturation time 30 min, renaturation temperature 4 ℃ and enzyme concentration 1 5×10 -4 g/mL. 1 0 mol/L (NH 4 ) 2 SO 4 showed a limited effect on the recovering of PPO activity. Using both 1 0 mol/L (NH 4 ) 2 SO 4 and 100 μmol/L CuSO 4 was the most effective method, and the renaturation system with both CTAB and β CD together showed a more favorable effect on the enzyme renaturation, than that with the single β CD.
出处
《应用化学》
CAS
CSCD
北大核心
2005年第1期9-13,共5页
Chinese Journal of Applied Chemistry
基金
国家自然科学基金资助项目(30270321)
关键词
多酚氧化酶
复性
盐酸胍
β-环糊精
溴化十六烷基三甲基铵
polyphenol oxidase,renaturation,guanidine hydrochloride, β cyclodextrin,cetyl trimethylammo nium bromide
