摘要
用RAPD和AFLP技术分析了14种兰属植物的遗传多样性。RAPD筛选出12个随机引物和AFLP 3对选择性引物组合均扩增出了丰富的多态性片段。分析结果按UPGMA类平均法进行聚类,所得到的RAPD和AFLP聚类结果基本一致,显示建兰与墨兰,寒兰与峨眉春蕙以及大雪兰与独占春之间的亲缘关系最近。
RAPDs and AFLPs were used to investigate the genetic variation of 14 species of Cymbidium.A great diversity was shown by 12 random primers and three primer combinations. UPGMA cluster analysis showed that the results from the RAPD and AFLP analysis were similar and the genetic distances were closest between C.ensifolium and C.sinense,C.kanran and C. faberi var.omeiense,C.mastersii and C.eburneum respectively.
出处
《实验生物学报》
CSCD
北大核心
2004年第6期482-486,共5页
Acta Biologiae Experimentalis Sinica
基金
浙江省自然科学基金(301406)
浙江省"151"人才基金
杭州市"131"人才基金。
