摘要
研究了高产莪术细胞悬浮系培养的条件及前体物质添加对挥发油合成的调控。结果表明:淡黄色颗粒状愈伤组织是建立高产细胞悬浮系的最佳供试愈伤组织;最佳培养基成分是MS培养基添加葡萄糖与蔗糖各15—30g/L(1:1),氮源为NH_4^+和NO_3^-,比例为1:3,总量为80mmol/L;激素组合为6-BA 3.0-5.0mg/L、2,4-D 1.0mg/L;光下培养10-15天再转入优化条件下的暗培养,可形成稳定的高产细胞悬浮系;其细胞周期中的最大细胞生长量及挥发油含量分别是248g/L和2.28%;前体物质泛酸钙、乙酸铵、乙酸钾的添加均可有效提高培养细胞合成挥发油的百分含量,其中乙酸铵最有效,在指数生长中期添加0.5mmol/L乙酸铵,挥发油的最高含量可达3.11%,产量为8.27g/L,分别是添加前的1.25倍及1.2倍。
The condition for high-yield suspension cell line and the precursors of volatile oil synthesis of Curcuma zedoaria(Berg.)Rosc were studied.The results showed that the light yellow particle callus was suitable for establishment of the high-yield suspension cell line.The optimum conditions for cell growth were MS medium added 15-30g/L glucose and 15-30g/L sucrose(1:1) as carbon source,the total concentration of 80mmol/L nitrogen source combined NH_4^+ with NO_3^- (1:3),hormones of 3.0-5.0mg/L 6-BA,1.0mg/L 2,4-D and dark culture after 10-15 days light culture.The 229g/L cell(FW)and 2.11% content of volatile oil were obtained in vitro.The addi- tion of precursors of calcium pantothenate,ammonium acetate and potassium acetate during the middle period of the cell suspension culture enhanced the volatile oil content respectively,and ammonium acetate was most effective among them.The highest yield of volatile oil obtained was 3.11% and 8.27g/L respectively,which was 1.25 and 1.2 times of the control group.
出处
《实验生物学报》
CSCD
北大核心
2004年第6期469-474,共6页
Acta Biologiae Experimentalis Sinica
基金
辽宁省科技攻关项目(01-21-08)。
