摘要
观察不同培养条件对富铁酵母的生长影响 ,研究其实验制备的方法。实验利用双吡啶比色法测定铁浓度。获得富铁酵母细胞培养的培养温度为 2 8℃ ,pH值为偏酸性 ,5～ 7左右 ,培养基添加铁盐为硫酸亚铁 ,搅拌速度为 2 0 0r/min ,接种量为 10 %。实验显示 :通过生物发酵技术 ,能用于制备富铁酵母。
In order to get the process of fermenting high iron yeast,its culturing factors were studied in all kinds of conditions in this experiment. The four different edible funguses were chosed to incubate in yeast milk to which added different iron compound concentrations,and then the biomass,yield and content of iron binging with Saccharomyces cerevisiae were observed.In this experiment the Serum concentration of iron was determined by spectrophotometer.Saccharomyces cerevisiae cell that was capable of binding high iron was exactly incubated at the temperature of 28 ℃,pH for 5～7 or so,ferrisulphas added to yeast milk,stirring speed to 200 r/min,and 10 percent of contents inoculated to yeast milk for the best.the high iron yeast was gotten by biotech fermenting technique .
出处
《中国草食动物》
CAS
2004年第6期3-6,共4页
China Herbivores
基金
甘肃省自然基金资助项目 (项目编号ZS0 0 1 -A2 1 - 0 36 -N)
