摘要
本文以[~3H]R1881为配体,建立了正常男性外阴部皮肤成纤维细胞雄激素受体(AR)的完整细胞检测法。实验表明外阴部皮肤成纤维细胞[~3H]R1881的特异结合具有高亲和力、低容量的特点及明显的雄激素结合的特异性,符合AR判定的基本条件。用Scatchard及单点分析法检测了18例我国正常成年男性包皮成纤维细胞[~3H]R1881的特异结合量,其值为6194±982结合位点/细胞((?)±SD)。该法取材比较容易,不需特殊仪器设备,是AR基础与临床研究的简便灵敏的方法。
A rapid, simple and reliable dispersed-whole cell method for assaying androgen receptors with l7α-methyl-~3H-trienolone (~3H-Rl881) in cultured human external genital fibroblasts was described. The apparent Ro and Kd of specific binding of those cells determined by Scatchard analysis were 9.6±2.0 fmol/l0~6 cells and 0.3±0.1nmol/L (X±s, n=G)respectively. Binding is linear with respect to cell number and shows those characteristics commonly attributed to androgen receptors:high affinity, low binding capacity, androgen specificity. In addition, mean androgen receptor level in cultured preputial skin fibroblasts in 18 normal adult men determined by this method were 6194±982 sites/cell.
