摘要
目的 :探讨重组人生长激素 (r- h GH )对人乳腺癌细胞株 MCF- 7(MCF- 7)增殖和分化的影响。方法 :以不同浓度 r- h GH分别加入处于对数生长期的 MCF- 7进行体外培养 ,4 8h后用流式细胞仪检测细胞周期的百分比构成变化 ,并与对照组进行比较。结果 :不同浓度的 r- h GH作用于 MCF- 7后 ,其细胞周期的 G0 ～ G1 期、S期、G2 ～ M期的细胞百分比变化 ,差异均无统计学意义 (P >0 .0 5 )。结论 :r- h GH对 MCF- 7的细胞周期无明显影响 ,既无促进 MCF- 7细胞增殖作用 ,也无抑制 MCF- 7细胞的作用。
Objective:To Study the effetc of recondinant human growth hormone (r hGH) on cell cycle of a human breast cancer cell line MCF 7.Methods:MCF 7 cells were cultured for 48 h in RPMI 1640 with r hGH for different concentrations,then cells were gathered and evaluated for cell cycle by flow cytometry (FCM).Result:Comparing with the control group,r hGH had no significant effect on the percentage of G 0～G 1,S and G 2～M phase of MCF 7 (P>0 05).Conclusion:The r hGH might not have effect on the cell cycle of MCF 7 cells.
出处
《广西医科大学学报》
CAS
2004年第2期195-196,共2页
Journal of Guangxi Medical University
