摘要
目的探讨胰腺移植再灌注后胰腺细胞凋亡发生的过程,以及Bax、Bcl2及Fas等调控基因的表达。方法65只SD大鼠随机分成7组假手术组,冷缺血2h组,冷缺血2h再灌注1、3、6、9、12h组。电镜观察胰腺组织的病理变化;采用缺口末端标记法(TUNEL)检测凋亡细胞;采用免疫组织化学方法检测胰腺组织中Bax、Bcl2及Fas蛋白的表达。结果胰腺移植后早期即可观察到细胞凋亡,凋亡的高峰期为再灌注后3h,凋亡指数(AI)为(95±29)%,P<001,再灌注6h组细胞凋亡有所减少,AI为(57±17)%,P<001,再灌注9h及12h组,AI分别为(36±16)%及(33±14)%,P<005。假手术组与冷缺血2h组均未见Bax、Fax蛋白表达。冷缺血再灌注1h组Bax(++)、Fas(+),未见Bcl2蛋白表达;至冷缺血再灌注3h组Bax及Fas表达均增强,分别为Bax(+++)、Fas(++),以后各组表达有所下降。Bcl2蛋白在假手术组、冷缺血2h组及冷缺血再灌注1h组均未见表达,冷缺血再灌注3h及以后各组表达为(+)。结论细胞凋亡是胰腺移植后的早期事件,Bax、Fas基因可能促进了胰腺细胞的凋亡,而Bcl2基因可能抑制细胞凋亡。
Objective To investigate apoptosis in acinar cells during posttransplant reperfusion, and the expression of Bcl 2, Bax, Fas Methods Pancreas transplantation was performed between male SD rats after 2 hours of cold ischemia Rats ( n =65) were divided at random into 7 groups: sham group, cold ischemia group (as controls), reperfused 1 h, 3 h, 6 h, 9 h, 12 h group The pathologic pancreatic tissue was observed by electron microscope; Apoptotic cells were stained by TUNEL technique; Expression of Bax、Bcl 2 and Fas protein in pancreatic tissue were detected by SABC immunohistochemical method Results Apoptotic cells were observed in early phase after pancreas transplantation The peak of apoptotic pancreas cells occurred 3 hours after reperfusion [AI ( 9 5± 2 9)%, P <0 01 vs controls, pancreas fixed after 2 hours of ischemia], with a steady decrease, at 6 hrs of [AI (5 7±1 7)%, P <0 01 vs controls], at 9 hrs [AI (3 6±1 6)%, P <0 05 vs controls] and 12 hours after reperfusion [AI (3 3±1 4)%, P <0 05 vs controls] No expression of Bax, Fas and Bcl 2 was detected in sham group and ischemia group Bcl 2 was expressed after three hours reperfusion The highest expression of Bax and Fas protein was in I/R3 group Conclusion Apoptosis is a very early event The peak of apoptotic pancreas cells occurred 3 hours after reperfusion The overexpression of Bax and Fas may accelerate the apoptosis, however, Bcl 2 may inhibit the apoptosis
出处
《中华普通外科杂志》
CSCD
北大核心
2004年第11期681-683,共3页
Chinese Journal of General Surgery
