摘要
目的 :探讨衰老对大鼠阴茎海绵体内皮细胞功能的影响。 方法 :利用YH 4压力传感器分别检测了青龄(5个月 )和老龄 (2 0个月 )两组大鼠阴茎海绵体内压 (ICP)在乙酰胆碱 (Ach)、硝普钠 (SNP)和A2 3187作用下的变化 ;并测定了两组大鼠阴茎海绵体一氧化氮合酶 (NOS)的活性。 结果 :青龄组基础ICP为 (9.4± 2 .3)mmHg(1mmHg=0 .133kPa) ,老龄组为 (7.2± 1.7)mmHg,二者间差异无显著性 (P >0 .0 5 )。在浓度分别为 10 -6、10 -5、10 -4mol/L的Ach作用下 ,两组大鼠ICP值间差异均有显著性 (P <0 .0 1)。当Ach浓度为 10 -4mol/L时 ,两组大鼠ICP值达到最高 ,青龄组为 (5 4 .8± 4 .2 )mmHg ,老龄组为 (40 .3± 2 .8)mmHg。A2 3187(10 μmol/L)可以进一步提高老龄组ICP值 ,由(40 .3± 2 .8)mmHg上升到 (5 6 .2± 4 .1)mmHg ,两者间差异有显著性 (P <0 .0 1) ;青龄组提高不明显 ,由 (5 4 .8± 4 .2 )mmHg上升到 (5 5 .8± 4 .7)mmHg ,两者间差异无显著性 (P >0 .0 5 )。在SNP(10 -4mol/L)作用下青龄组ICP值为(5 8.9± 4 .7)mmHg ,老龄组为 (5 1.7± 5 .3)mmHg ,两者间差异无统计学意义 (P >0 .0 5 )。两组大鼠阴茎海绵体内NOS的活性差异无统计学意义 (P >0 .0 5 )。 结论
Objective: To evaluate the effect of aging on the endothelial function of the penile corpus cavernosum in rats. Methods: The intracavernosal pressure(ICP) was compared in response to acetylcholine(Ach, endothelium-dependent vasodilator), sodium nitroprusside(SNP, an NO donor) and A23187(a calcium ionophore)in the young( 5 months old) and aged (20 months old) rats. In addition, the activity of nitric oxide synthase(NOS)in penile cavernosal tissues was examined. Results: Ach-mediated ICP was significantly attenuated from the maximum of ( 54.8± 4.2)in the young rats to ( 40.3± 2.8) mm Hg in the aged ones(Ach= 0.1 mmol/L),P< 0.01. The ICP to SNP( 0.1 mmol/L) was ( 58.9± 4.7)mm Hg in the young rats and ( 51.7± 5.3)mm Hg in the aged. No statistically significant difference was noted between the two groups, P> 0.05. The Ach-mediated ICP in the young rats was not significantly augmented from the maximum of ( 54.8± 4.2) to ( 55.8± 4.7)mm Hg in the presence of the calcium ionophore A23187(10 μmol/L),P> 0.05. However, A23187 significantly augmented Ach-mediated ICP in the aged from the maximum of ( 40.3± 2.8) to ( 56.2± 4.1) mm Hg,P< 0.01. Finally the activity of nitric oxide synthase was not significantly attenuated in either the aged or the young, P> 0.05. Conclusion: The endothelial function of the penile cavernosum declines with the advance of age. And endothelial dysfunction may play some role in the mechanisms of age-related erectile dysfunction.
出处
《中华男科学杂志》
CAS
CSCD
2004年第11期824-826,829,共4页
National Journal of Andrology
