摘要
背景与目的:研究硝酸羟胺对小鼠淋巴瘤L5178Y-3.7.2c tk+/-细胞tk基因突变的影响。材料与方法:使用硝酸羟胺对L5178Y细胞进行梯度染毒,再分别进行细胞毒性,细胞接种效率,相对存活率,相对悬浮生长率和突变频率测定。结果:随着硝酸羟胺的剂量增加,L5178Y细胞的相对存活率和悬浮生长率均下降。在硝酸羟胺50-500 μg/ml范围内可以诱导细胞琥基因的突变率达到自发突变率几倍至十几倍。结论:硝酸羟胺对L5178Y细胞具有明显的细胞毒性,并可以诱导tk基因突变。
BACKGROUND & AIM: To investigate the effects of Hydroxylammonium Nitrate (HAN) on the tk gene of mouse lymphoma cells. MATERIAL AND METHODS: L5178Y cells were treated with Hydroxylammonium Nitrate with different concentrations, to determine cytotxicity and mutation at different time phases. The numbers of positive wells were counted . The cell plating efficiency, relative suspension growth and mutation frequency were determined. RESULTS: The results showed that HAN (50-500 μg/ ml) induced tk gene mutation with mutation frequency 1 - 19 times higher than that of spontaneous mutation frequency of L5178Y cells and severe cytotoxic responses. CONCLUSION: Hydroxylammonium Nitrate can exert cytotocicity and mutagenic effects on tk geng in L5178Y cells.
出处
《癌变·畸变·突变》
CAS
CSCD
2004年第6期365-367,371,共4页
Carcinogenesis,Teratogenesis & Mutagenesis
基金
军队指令性课题(No.180.3160-O1L015)
国家杰出青年基金资助项目(No.30125037)
军队杰出青年基金资助项目(No.01J014)
