摘要
目的 :建立一种快速灵敏的气质联用测定血浆吗啡浓度的方法。方法 :以纳洛芬作内标 ,联合采用固相萃取和PFPA衍生化对血浆吗啡进行气质联用检测。吗啡和纳洛芬的特征质荷比分别为 4 14和 4 4 0 ,保留时间 5 3min和 6 1min。结果 :吗啡标准曲线在 0 0 2 2 - 2 2 μg·ml- 1 浓度范围内具有良好的相关性 (R2 >0 99) ,回收率范围为91% - 98% ,日内精密度 2 4 % - 6 0 % ,日间精密度 2 3% - 7 0 % ,最低检测线 1ng·ml- 1 。结论 :该方法简便可行、准确灵敏 ,可用于药物滥用检测。
Objective: To establish a rapid and sensitive method for detecting morphine concentration in plasma by GC/MS. Methods: The gas chromatography/mass spectrometry (GC/MS) was employed in this study with a combination use of solid-phase extraction (SPE) and derivatisation reaction with pentafluoropropionic anhydride (PFPA). Nalorphine served as an internal standard. The ion m/z 414 and 440 was chosen as the qualifying ion for morphine and nalorphine, with the retention time by 5.3 min and 6.1 min, respectively. Results: The linear relationship was excellent within the range of 0.022 to 2.2 μg·ml -1(R 2>0.99, n=5), and the limit of detection was 1 ng·ml -1. The relative recovery rate of morphine was 91%-98%. Within day and day-to-day coefficient of variation were 2.4%-6.0% and 2.3%-7.0%, respectively. Conclusion: The method is accurate, sensitive and convenient for the measurement of morphine concentration in plasma.
出处
《中国药物依赖性杂志》
CAS
CSCD
2004年第4期306-309,共4页
Chinese Journal of Drug Dependence
