摘要
本文采用光学显微镜和电子显微镜观察了球形芽孢杆菌C_3-41菌株感染致倦库蚊C(?)lexp(?)p(?)ens quinquefasciatus 幼虫的组织病理变化。实验结果表明,被感染幼虫的中肠上皮细胞出现膨大、脱落和瓦解,胃盲囊细胞也膨大和破坏。但围食膜在整个感染过程中保持完整。发现感染后3小时,细胞中的溶酶体增多增大,而线粒体在感染后6小时发生改变,其基质电子密度增高,嵴增宽,进而嵴变得模糊或消失,终至线粒体的瓦解。同时内质网也表现出膨大或破坏,基膜内褶膨大形成空泡。骨架肌肉组织及其细胞也产生一定程度的病变。本文还讨论了球形芽孢杆菌C_3-41毒素的作用机理。
The histopathological changes in the midgut of Culex pipiens quinquefaidants larvae trea-ted with Bacillus sphaericus strain C8-41 were observed with light and electron microscopes. The results showed that the epithelial cells of the midgut swelled, sloughed and disrupted sub-sequently, but the basement membrane of the cells and the peritrophic membrane remained in-tact. When the epithelial cells of the mid-gut were disrupted, the cells of caeca were also da-maged. The lysosomes in the epithelial cells increased in quantity and size three hours after infection, and the mitochondria were affected after six hours, including dilation of cristae, in-creasing electron density and subsequently leading to destruction. In the meantime, the endo-plasmic reticula swelled or disrupted, and the basal infolding of the basement membrane ex-panded to form vacuoles. Lesions appeared later in the skeletal muscles. The mechanism of larvicidal action of Bacillus sphaericus toxin is discussed.
出处
《昆虫学报》
CAS
CSCD
北大核心
1993年第1期34-38,共5页
Acta Entomologica Sinica
关键词
组织病理学
致乏库蚊
球形芽孢杆菌
Culex pipiens quinquefasciatus-Bacillus sphaericus strain C_(3)-41-histopathology
