摘要
目的 建立大剂量化疗时人血浆中甲氨蝶呤浓度RP-HPLC测定方法。方法 色谱条件为Kromasil C18柱,柱温40℃,流动相为乙晴:25 nmol·L-1磷酸溶液(17∶83,v/v),流速为1.0 mL·min-1,检测波长306 nm,样品用乙腈沉淀后取上层液N2吹干,残渣用氯仿-异丙醇(3:1,v/v)与20 mmol·L-1磷酸水溶液溶解,取上层液进样。结果 在0.04～128.00μg·mL-1浓度范围内,线性关系良好,r=1.000,最低检测浓度为0.01μg·mL-1,绝对回收率为83.6％,日内与日间RSD均低于5％(n=3)。结论 本方法线性范围宽、准确、简单,适用于MTX大剂量化疗时血药浓度监测,也可用于药物动力学研究。
OBJECTIVE To establish the RP-HPLC method to determine of methotrexate in chemical therapy with high dose in human plasma, METHODS Kromasil C15, column was used. The mobile phase consisted of acetonitrile: 25 mmol· L-1 phosphate acid solution (17:83, v/ v), at a flow rate of 1. 0 mL·min-1. The column temperature was 10℃ and UV wavelength was 306 nm. Samples were deposited by acetonitrile and dried by N2. The leftover was dissolved by chloroform-isopropanol (3 : 1 v/v) 20 mmol·L-1 phosphate acid solutions, and the resulting supernatant liquid was directly injected. RESULTS The minimal detectable methotrexate drug concentration was 0. 01 μg·mL-1. The calibration curve was linear within the range of 0. 04- 128. 00 μg·mL-1 (r= 1.000). The absolute recovery rate was 83. 6%. Inter-day and intra-day RSDs were lower than 5% (n = 3). CONCLUSIONS The method is accurate and simple with wide concentration range. It is suitable for the determination of methotrexate in chemical therapy with high dose in human plasma and pharmacokenetics study.
出处
《中南药学》
CAS
2003年第4期243-245,共3页
Central South Pharmacy
