摘要
目的 :建立可以对黏细菌蛋白质组进行阵列分离的双向电泳条件。方法 :以黏细菌模式菌株DK162 2为材料 ,不同方法制备DK162 2在营养性生长阶段的蛋白质组样品 ,双向电泳阵列菌体总蛋白。通过不同条件下电泳图谱的比较建立蛋白质组样品的制备方法和双向电泳的技术条件。结果 :两性离子去污剂CHAPS及还原剂的浓度是影响双向电泳图谱分辨力的重要因素 ;蛋白质组样品的处理必须在低温下快速完成 ,以避免蛋白降解。结论 :初步建立了黏细菌总蛋白质组的双向电泳条件 。
Objective To develop appropriate condit ions for profiling cell proteome of Myxococcus xanthus. Methods Total cellular proteins from Myxococcus xanthus DK1622 negetative cells were extracted and subjected t o two-dimensional gel electrophoresis under different conditions. Optimal cond i tions for sample preparation and 2-DE was established based on the comparison o f a series of protein patterns. Results The concentration of z witterionic deter gent CHAPS and reductant was decisive for obtaining good resolution of 2-DE pat t ern. Protein sample should also be processed as quickly as possible under low t emperature to prevent proteins from degradation. Conclusion Op timal conditions of 2-DE for proteomic analysis of Myxococcus xanthus has been established, which paves t he way for global profiling of protein expression patterns during the developmen t of this model organism.
出处
《南京铁道医学院学报》
2004年第6期373-376,共4页
Journal of Nanjing Railway Medical College
基金
国家自然科学基金资助项目 (30 370 775)
