摘要
本研究的目的就是利用细胞亲和力较好的胶原与壳聚糖复合 ,制备具有较好细胞亲和力的组织工程复合支架。在研究中采用冷冻诱导相分离的方法制备了壳聚糖 (Cs)和胶原溶胀液 (Co)质量比为 1∶2、1∶3、1∶4、1∶5的 4种成分的复合多孔支架 ,并对支架的孔结构、吸水性、孔隙率和溶胀性等特性进行了评价。结果显示 ,制备的复合多孔支架孔隙率均在 90 %以上 ;不同成分的支架孔径大小不同 ,胶原含量越高 ,孔径越大 ;支架内部孔隙连通 :残留的醋酸在超声波作用下用碱液中和并干燥后 ,支架浸入水中基本无溶胀 ,保持了原有的形貌。
To improve the cellular affinity of chitosan,chitosan-collagen porous composite scaffolds for tissue engineering were prepared in this study.The scaffolds with different ratios of chitosan to collagen 1∶2,1∶3,1∶4 and1∶5 were prepared by cryogenic induced phase separation (CIPS)respectively.The pore structure,bibulous ability,porosity and swelling ability were investigated.The results revealed that the porosities of porous composite scaffolds were all over 90%.The pore sizes varied with the composition of scaffolds which increased with the content of collagen.Because remaining acetic acid inscaffolds was neutralized with lye by ultrasonic oscillation,the scaffolds would not swell,and maintained their original morphology and shape.
出处
《化工时刊》
CAS
2004年第10期36-39,共4页
Chemical Industry Times
基金
江苏省高新技术研究项目 (BG2 0 0 1 0 33) .
