摘要
寻求早期诊断肝细胞癌微量转移的方法。方法 建立巢式逆转录聚合酶链反应(RT-PCR)技术,对44例原发性肝细胞癌(PHCC)患者外周血单个核细胞(PBMC)中甲胎蛋白信息核糖核酸(AFP-mRNA)进行了检测,并与慢性肝病患者和正常人进行对照。结果 44例PHCC中有27例检出AFP-mRAN,阳性率61.4％;10例肝硬化中2例阳性,占20％;肝炎及正常对照组均为阴性。结论 采用巢式RT-PCR法检测PHCC患者PBMC中AFP-mRNA是早期发现肝细胞癌微量转移的重要方法。
We attempted to find an early diagnostic method for micrometastasis of primary hepatocellularcarcinoma(PHCC). Methods Reverse transription polymerase chain reaction (RT-PCR) was established to detect AFP-mRNA of mononuclear cells in peripheral blood (PBMC) Results Out of 44 cases of PHCC , the AFP-mRNA was detected in PBMC(61. 4%) in 27 cases,in 2 of 10 cases of liver cirrhosis AFP-mRNA was found positive in PBMC(20%) whereas 5 cases of adult normal control were all negative for AFP-mRNA. Conclusions Detecting the AFP-mRNA in PBMC with RT-PCR may be an important method for early diagnosis of micrometastasis of PHCC.
