摘要
用免疫组织化学法,在冰冻切片上观察了不同胎龄组肝内前B细胞的形态、分布及其分化为B细胞过程中抗原的变化。在早期胎肝(9～20周)内有较多的前B细胞,大小不一,形态各异,但有共同的抗原,如IgM、BA-1、HLA-DR和TdT均为阳性。这些细胞多数分布在胎儿肝的窦周隙,少部分分布于窦内或血管周围。第13周后开始出现IgD和IgA阳性细胞,OKB-2和Leu 14阳性细胞随之大量增加,HLA-DR,Kappa和Lambda阳性细胞也相应增加。上述阳性细胞的出现提示B细胞进一步成熟。山于胎肝缺乏抗原刺激,B细胞不能发育为浆细胞。此外,B细胞在肝内的分化、成熟与T细胞关系不大,因为在缺少T细胞的情况下,B细胞仍在发育。说明B细胞在肝内的分化、发育主要依赖肝的微环境。本研究为选用第9～20周胎肝治疗再生障碍性贫血和B细胞缺乏症提供了依据。
The morphology and distribution of pre-B cells and the changes of antigens during the differentiation from pre-B to B cells in fetal livers of different gestational ages were studied by means of immunohistochemical technique. The results revealed that at earlyfetal stage(between 9 and 29 weeks), the liver contained numerous pre-B cells, which were different in shapes and sizes, but their phenotypic expression was identical, for instance, 1gM, BA-1, HLA-DR and TdT all appeared positive. Most of pre-B cells were scattered in perisinusoidal space, only a few of them were present in sinusoids or around the blood vessels. After the 13the week, 1gD and 1gA positive cells began to appear, and the number of OKB-2 and Leul4 positive cells was markedly increased subsquently. Meanwhile, the number of the HLA-DR, Kappa and Lambda positive cells was increased accordingly, which indicated that the B cells became more mature. In view of absence of antigenic stimulation, the B cells failed to develop into plasma cells. Moreover, it was observed that the differentiation and maturation of the B cells in the fetal liver seemed to be independent on the T cells, because the B cells continued to generate even if the T cells were absent at early stage of fetal liver. It showed that the differentiation and the development of the B cells in the fetuses were mainly dependent on the liver microenvironment. This study provided an evidence for treatment of aplastic anemia and agammaglobulinemia with fetal liver between 9 and 20 weeks old.
出处
《解剖学报》
CAS
CSCD
北大核心
1993年第4期400-405,共6页
Acta Anatomica Sinica
基金
福建省自然科学基金
关键词
前B细胞
免疫组织化学
胎肝
Pre-B cells
Monoclonal antibody
Immunohistochemistry
Fetal liver
