摘要
目的 探讨耳炎差异球菌与成人分泌性中耳炎的关系。方法 采用多重PCR的方法 ,检测 39例 (4 2耳 )成年分泌性中耳炎患者的中耳积液中耳炎差异球菌以及三种常见细菌 [肺炎链球菌 (S .pneumoniae)、流感嗜血杆菌 (H .influenzae)和卡他莫拉杆菌 (M .catarrhalis) ]的DNA ,就细菌DNA存在与成人分泌性中耳炎患者上呼吸道感染 (简称上感 )史、病程、积液性质和抗生素服用史之间的关系进行分析。结果 收集到的 4 2份中耳积液标本中有5份 (11.9% )检测到有耳炎差异球菌DNA存在。急性分泌性中耳炎者有 1例 (3.6 % )测得耳炎差异球菌DNA存在 ,慢性者则有 4例 (2 8.6 % ) ,两者差异有显著性意义。浆液性中耳积液有 3例 (13.0 % )测得耳炎差异球菌DNA存在 ,粘液性中耳积液则有 2例 (10 .5 % )为阳性 ,两者差异有显著性意义。抗生素使用史对耳炎差异球菌DNA检出率的影响无统计学意义。结论 细菌参与部分成人分泌性中耳炎的发病 ,耳炎差异球菌可能是其致病菌之一 ;
Objective To investigate the relationship between Alloiococcus otitidis (A. otitidis) and Secretory Otitis Media (SOM) in adults.Methods The study included 39 adult patients suffering from SOM and 42 samples (middle ear effusion, MEE)were collected. We detected the four organisms(A. otitidis ,S. pneumoniae, H.influenzae and M. catarrhalis) by mutiplex PCR, for comparing the bacterial findings in the patients with or without cold history, with or without antibiotic treatment, with effusion of different characters and durations by chi-square test. Results The DNA of A. otitidis was detected from 5 MEEs(11.9%).To acute SOM, only 1 sample was PCR-positive for A. otitidis; and to chronic SOM,4 samples were positive. There was a significant difference between them. There were 3 serous MEEs and 2 mucous MEEs, which were PCR-positive for A. otitidis, and there was a significant difference between them. Antibiotic therapy didn't contribute to PCR-positive for A.otitidis. Conclusion There is A. otitidis in the MEEs of adult SOM and it may be a pathogenic factor in SOM. A. otitidis may play an important role in chronic SOM.
出处
《听力学及言语疾病杂志》
CAS
CSCD
2004年第6期396-398,共3页
Journal of Audiology and Speech Pathology
基金
广州中山大学附属第二医院科研基金项目 (编号 F0 0 2 0 0 3 0 16)
