摘要
目的评价组织隔离法与机械活动法在建立兔胫骨萎缩型与肥大型骨不连模型中的作用。方法将12只体重为3～4.5kg的新西兰大白兔随机分成A、B两组,A组于胫骨中段截骨,两断端套接1cm硅胶管,单侧外固定器固定,保持两断端间距2mm,8周取出硅胶管,观察组织隔离法构建萎缩型骨不连模型的效果。B组于胫骨中段截骨后采用2枚1mm克氏针行髓内松动固定,被动活动断端200次/天,持续1个月,观察机械活动法构建肥大型骨不连模型的效果。结果A组中所有动物在硅胶管取出后4周,无1例断端出现骨愈合表现,X线片显示良好萎缩型骨不连的复制。B组中所有动物在6周内截骨端出现延迟愈合,部分伴有畸形,骨断端有大量肥大骨痂形成。结论硅胶管组织隔离法是复制兔胫骨萎缩型骨不连模型的有效方法,而采用被动机械活动复制兔胫骨肥大型骨不连模型的方法尚需进一步研究。
Objective To select an effective method for establishment or reproduction of atrophic and hypertrophic non union model of tibial diaphysis in rabbits. Methods 12 New Zealand white rabbits of 18 weeks with body weight ranging from 3 to 4.5 kg were randomly distributed into 2 treatment groups. In Group A, the tissue isolation technique was applied so as to establish the atrophic non union model. After a transverse osteotomy of the middle third of the tibial shaft was made, the periosteum on either side of the fracture site was excised and the marrow was removed by curettage. The either end of the fracture was covered by 1 cm silastic tube and the fracture was fixed with a Hoffman unilateral external fixator. The fracture gap was maintained to 2 mm. 8 weeks later, the silastic tube was removed. In Group B, the manipulation of mechanical mobilization was used in order to reproduce the hypertrophic non union model. The surgery procedure was similar to that of Group A. However, the silastic tube was not placed over the osteotomy end and a K wire was introduced as an intramedullary nail for fixation. Passive motion of the fracture site was performed with 200 times/d for 1 month. The non union of osteotomy was evaluated using plain radiograph. Results None of the osteotomies had healed at the operative site following removal of the silastic tubes after an interval of 4 weeks in all the rabbits of Group A. The radiographs displayed the consistent reproduction of atrophic non union. In Group B, all the rabbits were observed to have delayed union and deformity healing in 6 weeks. There were a large number of hypertrophied callus at the fracture site. Conclusions The tissue isolation technique seems to be a consistent and available method to reproduce atrophic non union model of the rabbit tibial diaphysis. The method of passive mechanical mobilization to create hypertrophic non union model in rabbits needs to be investigated further.
出处
《中华创伤骨科杂志》
CAS
CSCD
2004年第11期1261-1264,共4页
Chinese Journal of Orthopaedic Trauma
关键词
骨不连
动物模型
兔
胫骨
Fracture nonunion
Theoretical model
Rabbit
Tibial
