摘要
目的 比较玻璃化法冻融小鼠桑葚胚期、囊胚早期和囊胚期胚胎后,胚胎存活及继续发育的能力。方法应用6 mol/L乙二醇和1 mol/L蔗糖的玻璃化冷冻液,冻融小鼠142个桑葚胚期、135个囊胚早期和148个囊胚期胚胎,观察冻融后胚胎存活及继续发育的情况。结果 小鼠桑葚胚期、囊胚早期和囊胚期胚胎的存活率分别为88.0％、73.3％和60.1％,囊胚孵出率分别为73.9%、61.5％和49.3％。桑葚胚期的胚胎存活率及囊胚孵出率均高于囊胚早期,囊胚早期高于囊胚期,各期胚胎存活率、囊胚孵出率比较,差异均有显著性(P<0.05)。结论 玻璃化法冻融桑葚胚期的效果好于囊胚早期及囊胚期,桑葚胚期是卵裂期后胚胎玻璃化冻融的最佳阶段。
Objective To compare the survival and developmental potential of mouse morula, early blastocysts and blastocysts cryopreserved by vitrification. Methods One hundred and forty-two mouse morula, 135 early blastocysts and 148 blastocysts were cryopreserved by 6 mol/L ethylene glycol and 1 mol/L sucrose vitrification solutions. The survival rates and blastocysts hatching rates after thawing were observed. Results The survival rates of morula, early blastocysts and blastocysts groups were 88. 0% ,73. 3% ,and 60. 1% respectively. The blastocyst hatching rates were 73. 9% , 61. 5% ,and 49. 3% respectively. Both the survival rates and blastocyst hatching rates in morula group were higher than those in early blastocysts group (P < 0. 05 ) , and they were higher in early blastocysts group than in blastocysts group ( P < 0. 05 ) . Conclusions In these three embryo stages, the survival and developmental potential of mouse morula cryopreserved by vitrification are better than those of early blastocysts and blastocysts. Morula is likely the most feasible stage for embryo vitrification.
出处
《中华妇产科杂志》
CAS
CSCD
北大核心
2004年第8期526-528,共3页
Chinese Journal of Obstetrics and Gynecology
