摘要
目的 观察大豆磷脂 (soybeanphospholipid ,SPL)治疗大鼠肝纤维化的疗效。 方法 采用复合病因诱导大鼠肝纤维化模型 ,应用大豆磷脂进行治疗 ,检测血清谷丙转氨酶 (ALT)、谷草转氨酶 (AST)以及肝匀浆丙二醛 (MDA)和羟脯氨酸 (HOP)。结果 ①肝纤维化模型组血清ALT、AST明显升高 ,大豆磷脂治疗组升高较少 ,两组之间有显著性差异 (P <0 0 1)。②肝纤维化模型组肝匀浆MDA显著升高 ,大豆磷脂组显著低于模型组 (P <0 0 1) ,而与正常组相比无显著差异 (P >0 0 5 )。③肝纤维化模型组肝匀浆HOP比大豆磷脂组高得多 ,两组之间有显著性差异 (P <0 0 1)。④肝组织行HE染色 ,观察到Control组肝小叶结构完整 ,无变形、坏死及纤维组织增生。Model组正常肝小叶被破坏 ,肝板排列紊乱 ,肝细胞中充满脂滴 ,可见到程度不等、范围不一的肝细胞坏死区。门脉区有大量增生的胶原纤维 ,向肝小叶内伸展 ,纤维组织相互环绕而成大量假小叶 ,纤维间隔较宽。SPL组这些改变明显较轻。
Objective To investigate preventive effect of soybean phospholipid (SPL) on liver fibrosis in the rat.Methods Multiple factors-induced liver fibrosis model was established in rats and they were treated with SPL. Then serum lever of ALT and AST, MDA and HOP in liver tissues were determined to reveal the extent of liver damages.Results ① ALT and AST in the serum were obviously higher in Model group than in SPL group. ② MDA in the liver was lower in SPL group than in Model group(P<0.01). MDA in the liver was not as high in SPL group as in the Control. ③ HOP in the liver was much higher in Model group than in SPL group(P<0.01).④ The liver tissue was HE dying. In Control group the structure of the liver was normal without deforming, necrosis and fiber proliferation. In the Model group the liver nodules was destroyed with cells chaos, many fat drops and many necrosis regions in the liver cells. There was much fiber proliferation in the portal regions, the fiber tissues formed many artificial nodules and fiber septums was wider. But in control group these changes were much lighter.Conclusions SPL can attenuate liver damages,protect against lipoperoxidation and attenuate liver fibrosis induced by multiple factors.
出处
《锦州医学院学报》
2003年第6期22-24,共3页
Journal of Jinzhou Medical College
