摘要
根据蔷薇科S RNase基因 (S基因 )高度保守区C2和RC4区设计一对特异引物PruC2和PruC4R ,对甜樱桃品种的基因组DNA进行S基因特异PCR扩增。克隆S基因的扩增片段 ,核酸序列在GenBank上搜索 ,确定了4种S基因的核酸序列和大小。结果表明 ,在琼脂糖凝胶上位置相同的扩增带其核酸序列相同 ,是同一种S基因。4种S基因扩增片段的大小分别是 :S1为 6 77bp ,S3 为 76 2bp ,S4为 94 5bp ,S6为 4 5 6bp。参试的自交不亲和品种的S基因型分别是 :红灯、红艳、早红宝石和先锋相同 ,为S1S3 ;抉择、红丰和那翁相同 ,为S3 S4;大紫为S1S6;长把红为S1S4;养老为S3 S6;自交亲和品种外引 7号和斯太拉为S3 S4′。
This report identified S-RNase genes (S genes) of sweet cherry (Prunus avium L.),presented the sequences of S genes by using a pair of specific primers PruC2 and PruC4R based on the conserved regions C2 and RC4 of Rosaceous S-RNase genes and researched the S gene specific products from the genomic DNAs of different cultivars in which most of the S genotypese were unknown.The bands of PCR were cloned and their sequences were compared in GenBank.Four S genes were defined and the conclusion was made that all the same bands from PCR in the agarose gel had the same length and sequence of nucleic acid and were the same kind of S gene.The lengths of the amplified S genes are as follows:S_1 is 677 bp,S_3 762 bp,S_4 945 bp,S_6 456 bp.The S genotypes (S gene genotypes) of the tested self-incompatible cultivars were identified as follows: 'Hongdeng','Hongyan' and 'Early ruby',as same as 'Van',were S_1S_3; 'Jueze','Hongfeng' and 'Napoleon' were S_3S_4; 'Dazi' was S_1S_6; 'Changbahong 'was S_1S_4; 'Elton' was S_3S_6.The self-compatibile cultivars 'Waiyin No.7' and 'Stella' had the same S genotypes S_3S_4′.
