摘要
以‘西子绿’ב翠冠’梨组合的2个F1代品种‘初夏绿’与‘翠玉’为材料,利用S基因PCR-RFLP(聚合酶链反应-限制性片段长度多态性)检测技术与PCR产物转质粒测序技术鉴定其基因型,并综合应用花粉原位萌发动态的荧光显微观察与田间授粉试验研究其杂交亲和性强度。结果表明:‘初夏绿’与‘翠玉’的S基因型皆为S3S4;‘初夏绿’和‘翠玉’的自交不亲和强度中等,二者相互间授粉不完全亲和,而与‘翠冠’间的相互授粉均表现为完全亲和。
By using polymerase chain reaction-restriction fragment length polymorphism （PCR-RFLP） based S-gene identification system and DNA sequencing technology, the S genotypes of ＇Chuxialv＇ and ＇ Cuiyu＇ （Pyrus pyrifolia Nakai. cvs. , two of the F1 progenies selected from ＇ Xizilti＇ × ＇ Cuiguan＇ ） were confirmed as S3S4, respectively. The results of fluorescence microscopic observation of pollen germination dynamics in situ and artificial pollination experiment showed that, both ＇Chuxialv＇ and ＇Cuiyu＇ had middle strength of self-incompatibility, but the cross-compatibility between them were incomplete; the self-incompatibility strength of ＇Cuiguan＇ was high, while its cross-compatibility with ＇ Chuxialv＇ and ＇ Cuiyu＇ was observed.
出处
《浙江大学学报（农业与生命科学版）》
CAS
CSCD
北大核心
2012年第2期132-138,共7页
Journal of Zhejiang University:Agriculture and Life Sciences
基金
国家现代农业(梨)产业技术体系资助项目(CARS-29-04)
浙江省重点科技创新团队资助项目(2009R50033)
