摘要
利用本室克隆的DMRT/Dmrt基因以及多个基因数据库中收集的DMRT/Dmrt基因全 (或部分 )序列 ,构建了DMRT/Dmrt基因的系统发生树 ,DMRT/Dmrt基因明显地聚为 7类 (DMRT/Dmrt 1～DMRT/Dmrt7)。以已克隆的斑马鱼Dmrt 1基因序列及数据库中人和小鼠的DMRT/Dmrt基因序列 ,结合基因组数据的比对分析 ,发现脊椎动物DMRT/Dmrt基因家族成员在基因组中的定位呈现高度保守的同线性 ,主要集中于两大同线群 ,即DMRT/Dmrt 1～ 3和DMRT/Dmrt 5～ 6。
Vertebrates contain a family of genes related to the Drosophila doublesex and C.elegans mab-3 genes,which encode transcription factors including a DNA-binding motif,DM domain.Evolution and function of different DMRT genes of vertebrates have not been understood yet,although some DM proteins are involved in sex determination,sexual differentiation and early embryonic development among different phyla.By genomic (analysis) of zebrafish and rat DMRT genes,all protein sequences of the vertebrate DMRTs were searched from gene databases and aligned.Phylogenetic tree of all these DMRT genes was reconstructed and evaluated by Bootstrap method.These DMRT genes were clustered into seven subfamilies.Results from analysis of gene structure and cluster organization of DMRT genes showed that synteny of DMRT genes of vertebrates were highly conserved among human,mouse,rat,fugu,medaka and zebrafish,with two syntenic groups,DMRT 1～3 and DMRT 5～6.
基金
国家自然科学基金资助项目 (批准号 :3 0 14 0 0 13 )~~
