摘要
单核苷酸多态性 (singlenucleotidepolymorphism ,SNP)是指DNA序列上的单个碱基变异 ,它具有分布广、多态信息量大、易于检测和统计分析等优点 ,被称为继RFLP和微卫星标记后的第三代基因遗传标记。单核苷酸多态性是等位基因间序列差异最为普遍的类型 ,可作为一种高通量的遗传标记。已建立PCR扩增目标序列及其产物测序和电子SNP(eSNP)等多种发现和检测SNP的方法。大豆等作物也已开展了SNP分析。
Single nucleotide polymorphism (SNP) refers to the change of single nucleotide in DNA sequence. Because of its high density in genomes and easy in detection and analysis statistically, SNP can be used in genetic linkage map construction and QTL mapping. Single nucleotide polymorphism( SNP) is the most common type of sequence difference between alleles,which can be used as a kind of high-throughput genetic marker. Several different routes were developed to discover and identify SNP. These included the direct sequencing of PCR amplicons, electronic SNP(eSNP) and so on. SNP assays were made in many crop species such as soybean. SNP analysis was broadly used in the field of plant gene mapping, integration of genetic and physical maps, DNA marker-assisted breeding and functional genomics.
出处
《安徽农业科学》
CAS
2004年第5期1000-1002,共3页
Journal of Anhui Agricultural Sciences
