摘要
目的 了解福建省HTLV Ⅰ毒株的亚型及进化起源。方法 用PCR的方法扩增出福建 5株样品的Env区及LTR区基因 ,测序后与世界各地的代表株进行比较、分析 ,构建进化树。结果将 5株样品与WHP、MT2、TAC1进行比较 ,其Env区核苷酸变异率在 0 .17%～ 1.2 3% ,LTR区在 0 .2 8%～ 1.2 6 % ,无缺失、插入。结论 获自福建的分离株与日本和我国台湾、汕头的代表株最接近 ,属于HTLV ⅠA亚型 (Cosmopolitan)的Transcontinental亚群 ,它们来源于共同的祖先。
Objective To identify the HTLV-Ⅰ subtype in F ujian province and evolution origin. Methods The gene of proviral envelope region and long terminal repeat region from five HTLV-Ⅰ stra ins in Fujian were amplified by PCR and then sequenced. They were compared with other isolates from different regions of the world. The phylogenetic tree was co nstructed by PHYLIP software. Results Sequencing and comp arison of Env fragment with the same fragment of HTLV-Ⅰ isolates from Japan, T aiwan and Shantou of China, showed a variability range from 0.17% to 1.23%, and the LTR fragment was from 0.28% to 1.26%. Without deletion nor insertion. Conclusion The strains from Fujian province were very closed t o the isolates from Japan, Taiwan and Shantou of China. It belongs to transconti nental subgroup of HTLV-Ⅰ subtype A (cosmopolitan)with commom origin of evol ution. [
出处
《中华微生物学和免疫学杂志》
CAS
CSCD
北大核心
2004年第9期715-718,共4页
Chinese Journal of Microbiology and Immunology
基金
福建省卫生厅青年科技基金资助项目 (2 0 0 2 1 18)
