摘要
目的 建立一种简单、实用的小鼠膀胱肿瘤动物模型。方法 将绿色荧光蛋白 (GFP)表达质粒导入膀胱癌细胞株BTT T739,筛选出高表达GFP的细胞克隆并配置成细胞悬液。将一定浓度肿瘤细胞悬液经雌性同系小鼠尿道注入膀胱壁粘膜层。荧光显微镜观察肿瘤浸润生长及微转移 ,常规病理切片观察。与同时建立的经腹膀胱壁内注射肿瘤模型进行对比。结果 经尿道种植膀胱肿瘤细胞 (4× 10 5个 ) 1周后可见膀胱粘膜层肿瘤细胞生长 ,而经腹膀胱壁内注射产生的肿瘤多在膀胱浆膜层及肌层。随时间延长膀胱内肿瘤逐渐增大 ,并出现血尿及远处转移的表现。结论经尿道膀胱壁注射肿瘤细胞建立膀胱癌模型的方法简单、实用、迅速。用该方法建立的模型更符合人类膀胱肿瘤的病理过程 ,有望成为研究膀胱肿瘤更为有效的工具。
Objective To establish a simple and useful bladder cancer mouse model. Methods BTT-T739 cells, a mouse bladder transitional cell carcinoma cell line, were tranfected with GFP plasmid to screen stable GFP expressing clones. The latter were injected in mucous membrane of bladder wall though urethra of female mouse. The tumor cell growth, invasion and metastasis were observed and evaluated though fluorescent microscopy. The findings were compared with outcome of pathology and HE staining on routine paraffin sections. The outcomes also were compared with those of the model which were implanted though abdomen. Results One week after in situ transplantation of bladder tumor cells (4×105) though urethra, tumor occurred in the mucous membrane of bladder wall, while the tumor which were implanted though abdomen occurred in the chorion and muscle membrance. Over the time, the tumor growth, hematuria and metastasis were easily observed. Conclusion The new method of implanting orthotopic mouse bladder tumor is simple and useful, and the established bladder neoplasm model has the same biological behaviors as the human primary bladder malignant neoplasms.
出处
《华中科技大学学报（医学版）》
CAS
CSCD
北大核心
2004年第5期586-588,共3页
Acta Medicinae Universitatis Scientiae et Technologiae Huazhong
