摘要
目的 :观察人参白虎汤不同配伍组别及活性部位对链脲佐菌素 (STZ)诱导的糖尿病大鼠肥大心肌中葡萄糖转运蛋白 4 (GluT4)基因表达的影响。方法 :雄性SD大鼠 ,除正常对照组外 ,其余大鼠ipSTZ(6 0mg/kg)造成糖尿病模型后 ,随机分为 9组 ,治疗组用人参白虎汤不同配伍组别及活性部位治疗共 75d。动物处死后取心肌组织 ,提取心肌组织总RNA ,逆转录得到cDNA第一链 ,以此为模板进行PCR扩增 ,利用聚丙烯酰胺凝胶电泳和DNA银染技术分析心肌组织中GluT4的基因表达。结果 :与正常对照组相比 ,STZ诱导的糖尿病大鼠肥大心肌中 ,GluT4基因表达下调。经人参白虎汤不同配伍组别 (除人参知母组外 )及其活性部位治疗后 ,心脏指数和心室指数与模型组相比均明显下降 ,GluT4mRNA表达上调。结论 :人参白虎汤及其活性部位能上调STZ诱导的糖尿病大鼠心肌中GluT4mRNA表达 。
AIM:To observe the effects of different compatible groups and some active parts from Renshenbaihu Decoction on the level of mRNA expression of Glu T 4 gene in diabetic rats' hypertrophic myocardium induced by streptozotocin.METHOD:Male Sprague-Dawley diabetic rats induced by streptozotocin were randomly divided into nine groups.They were treated with different compatible groups,and some active parts from Renshenbaihu Decoction for 75 days.Total RNA was extracted from myocardium tissue of rats and the gene of Glu T 4 and β-actin were amplified by RT-PCR. Silver staining and polyacrylamide gels were used to detect the level of mRNA expression of Glu T 4 gene in myocardium tissue.RESULT:The level of mRNA expression of Glu T 4 gene in hypertrophic myocardium tissue induced by STZ was downregulated.After being treated with different compatible groups(except the group of Rhizoma anemarrhenae compatible with Radix Ginseng) and some active parts from Renshenbaihu Decoction,the indexes of heart and ventricle were lowered and the level of mRNA expression of Glu T 4 gene was upregulated.CONCLUSION: Renshenbaihu Decoction and its active parts have the effects to upregulate the level of mRNA expression of Glu T 4 gene in diabetic rats' hypertrophic myocardium induced by streptozotocin.
出处
《中国药科大学学报》
CAS
CSCD
北大核心
2004年第5期460-465,共6页
Journal of China Pharmaceutical University
关键词
人参白虎汤
糖尿病
GLUT4
心肌病变
Renshenbaihu Decoction
Diabetes mellitus
Glu T4
Cardiomyopathy
