摘要
目的 :建立复方地巴唑氢氯噻嗪胶囊中 5种有效成分的含量的HPLC测定方法。方法 :采用Hy persilODS色谱柱 (2 0 0mm× 4 0mm ,5 μm) ;0 14 %己烷磺酸钠溶液 (含 1%冰醋酸 ,0 1%三乙胺 ) 甲醇 (1∶1)为流动相 ,用前经 0 4 5 μm的过滤膜 ,超声脱气 ;流速为 1 0ml/min ;紫外检测波长为 2 5 8nm ;柱温为 35℃ ,进样量 2 0μl。 结果 :氢氯噻嗪、地巴唑、磷酸氯喹、氯氮、盐酸异丙嗪检测限分别为 0 4 ,0 5 ,0 3,0 1,0 2ng ,线性范围分别为 1 0 0～ 98 80 ,0 96～ 96 0 0 ,1 0 6～ 10 6 4 0 ,0 86～ 85 6 0 ,0 84～ 83 6 0 μg/ml,平均回收率均在 98 6 5 %以上 ,精密度的相对标准偏差小于 0 5 7%。结论 :该方法简单、快速、灵敏度高、重复性好。
AIM:To establish the method for determining five ingredients in Compound Bendazol and Hydrochlorothiazide Capsules by RP-HPLC METHOD:The separation was performed on a Hypersil ODS2 column(200 mm×4 0 mm,5 μm) Mobile phase composed of methanol-0 14% heptanesulfonic acid sodium salt solution containing 1% acetic acid and 0 1% triethylamine(1∶1) was degassed ultrasonically before use Each component of the mobile phase was filtered through a 0 45 μm membrane The separation was performed at 35 ℃ and the flow-rate was 1 0 ml/min Injection volume was 20 μl All peaks were monitored at UV 258 nm RESULT:The detection limits of hydrochlorothiazide,bendazol,chloroquine phosphate,chlordiazeposide and promethazine hydrochloride were 0 4,0 5,0 3,0 1,0 2 ng respectively The linear ranges were 1 00～98 80,0 96～96 00,1 06～106 40,0 86～85 60,0 84～83 60 μg/ml respectively The average recovery of all compounds was over 98 65 % RSD was less than 0 57% CONCLUSION:The method was simple,rapid,sensitive and reproducible
出处
《中国药科大学学报》
CAS
CSCD
北大核心
2004年第5期446-448,共3页
Journal of China Pharmaceutical University
