摘要
AIM: To investigate the association between Chinese patients with autoirnrnune hepatitis (AIH), primary biliary cirrhosis (PBC) and the polyrnorphisrns of cytotoxic T lymphocyte-associated antigen-4 (CTLA-4) gene promoter (-318) and exon 1 (+49). METHODS: CTLA-4 promoter (-318 T/C) and exonl (+49A/G) polymorphisms were genotyped via restriction fragment length polymorphisrn methods in 62 Chinese AIH patients,77 Chinese PBC patients and 160 healthy controls. RESULTS: We found a significant association in CTLA-4 gene exonl 49 A/G polyrnorphisrn between PBC patients and controls (P = 0.006) and the frequency of G alleles was significantly increased in comparison with controls (P = 0.0046, OR = 1.8). We also found the frequency of C alleles in promoter -318 was significantly increased in AIH patients compared with controls (P= 0.02, OR = 0.41). Although the genotype distribution of the CTLA-4 exon 1-promoter gene was not significantly different between AIH and PBC patients and controls, the occurence of GG-CC was increased in two groups of patients (AIH: 32.3%, PBC: 37.7%, control: 22.5%). CONCLUSION: Polyrnorphisrns of CTLA-4 gene probably confer susceptibility to AIH and PBC in Chinese population.
AIM:To investigate the association between Chinese patients with autoimmune hepatitis(AIH),primary biliary cirrhosis(PBC)and the polymorphisms of cytotoxic T lymphocyte-associated antigen-4(CTLA-4)gene promoter (-318)and exon 1(+49). METHODS:CTLA-4 prornoter(-318 T/C)and exonl(+49A/G) polymorphisms were genotyped via restriction fragment length polymorphism methods in 62 Chinese AIH patients, 77 Chinese PBC patients and 160 healthy controls. RESULTS:We found a significant association in CTLA-4 gene exonl 49 A/G polymorphism between PBC patients and controls(P=0.006)and the frequency of G alleles was significantly increased in comparison with controls (P=0.0046,OR=1.8).We also found the frequency of C alleles in promoter-318 was significantly increased in AIH patients compared with controls(P=0.02,OR=0.41). Although the genotype distribution of the CTLA-4 exon 1- promoter gene was not significantly different between AIH and PBC patients and controls,the occurence of GG-CC was increased in two groups of patients(AIH:32.3%,PBC: 37.7%,control:22.5%). CONCLUSION:Polymorphisms of CTLA-4 gene probably confer susceptibility to AIH and PBC in Chinese population.
