摘要
目的观察久强脑力清 (JNQ)含药血清对人脐静脉内皮细胞 (HUVECs)分泌一氧化氮 (NO)和内皮素 (ET 1)的影响 ,探讨其对HUVECs血管活性分子分泌的调节作用。方法取原始JNQ含药血清体积分数的 80 %、40 %、2 0 %、10 %和 5 %作用于原代培养的HUVECs 2 4h后 ,分别检测上清中的NO和EI 1的含量。结果不同浓度的JNQ含药血清作用组的NO、ET 1含量与正常血清对照组比较增加 (P <0 0 5 ) ;NO/ET 1比值随着含药血清浓度的增加 ,比正常血清组增高 (P <0 0 5 )。结论JNQ含药血清通过提高HUVECs分泌NO和ET 1,具有保护内皮细胞功能和维持NO/ET
ObjectiveTo investigate the effect of Jiuqiang Naoliqing(JNQ) containing serum on secretion of nitric oxide(NO) and endothelin 1(ET 1) in human umbilical vein endothelial cells(HUVECs), in order to explore the effect of JNQ on regulation of vascular active factors in HUVECs.MethodsThe HUVECs were explored to different volume fractions of JNQ containing serum after being isolated and cultured. The levels of cultured medium of NO and ET 1 were measured.ResultsThe cultured medium content of NO and ET 1 in different volume fractions of JNQ containing serum was significantly increased compared with normal serum control ( P <0 05), while the ratio of NO to ET 1 were increased in comparison with normal control ( P <0 05).ConclusionJNQ can be promoted secretion of NO and ET 1 in HUVECs, preserving endothelial function, and maintaining the balance of NO/E 1.
出处
《中国康复理论与实践》
CSCD
2004年第9期522-523,共2页
Chinese Journal of Rehabilitation Theory and Practice
关键词
久强脑力清
人脐静脉内皮细胞
一氧化氮
内皮素
Jiuqiang Naoliqing
human umbilical vein endothelial cells(HUVECs)
nitric oxide(NO)
Endothelin-1(ET-1)
