期刊文献+
任意字段
题名或关键词
题名
关键词
文摘
作者
第一作者
机构
刊名
分类号
参考文献
作者简介
基金资助
栏目信息

定量实时RT-PCR检测非霍奇金淋巴瘤端粒结合因子2 mRNA的表达 被引量：2

Quantitative detection of telomere binding factor 2 gene expression in non-Hodgkin lymphoma with a real-time reverse transcription-polymerase chain reaction assay
暂未订购
导出
摘要 目的 :建立定量实时 RT- PCR,研究非霍奇金淋巴瘤 (NHL)患者肿瘤组织端粒结合因子 2 (TRF2 )m RNA表达。方法 :用 RT- PCR扩增目标基因 c DNA,电泳后切胶、纯化作为标准品 ,建立待测基因标准曲线 ;用实时 RT- PCR测定 32例 NHL和 8例反应性淋巴结炎患者淋巴组织 TRF2的表达。结果 :实时 RT- PCR标准曲线中 ,模板 c DNA含量和扩增时产生的荧光信号相关系数为 0 .996。实时 RT- PCR终点产物凝胶电泳的基因条带灰度值和模板 c DNA含量的相关系数为 0 .779(P<0 .0 5 )。在 NHL患者中 ,滤泡型淋巴瘤、套细胞淋巴瘤和弥漫性大 B细胞淋巴瘤 TRF2 m RNA表达量分别为 (2 2 .94 3± 9.4 2 4 ) amol、(2 3.181± 5 .983) amol和 (18.339± 7.910 ) amol,与良性反应性淋巴结炎组织 [(2 1.796± 4 .80 0 ) amol]相比无显著性差异 (P>0 .0 5 )。但 Burkitt淋巴瘤 TRF2 m RNA表达量为 (33.170± 12 .84 1) amol,显著高于良性反应性淋巴结炎组织和其他类型恶性淋巴瘤 (P<0 .0 5 )。结论 :定量实时 RT- PCR能精确测定目标基因 m RNA表达。在 NHL中 ,Burkitt淋巴瘤 TRF2 m RNA表达量明显高于滤泡型淋巴瘤、套细胞性淋巴瘤、弥漫性大 B细胞淋巴瘤。 Objective: To detect the expression levels of telomere binding factor 2 (TRF2) mRNA in tumor tissue of non Hodgkin lymphoma (NHL) patients using quantitive real time RT PCR. Methods: The target gene mRNA was amplified with RT PCR, then was sequentially electrophoresed and purified as standards, and the standard curves of gene expression were established. The expression levels of TRF2 mRNA of lymphoid tissue from NHL and reactive lymphoadenopathy were detected with real time RT PCR. Results: The correlation coefficient was 0.996 between the amount of template cDNA and the intensity of fluorescence signal when gene expression standard curves were established. The correlation coefficient of template cDNA amount and grey density of bands derived from gel electrophoresis of real time RT PCR final products was 0 779 ( P <0 05). Of all NHL patients, expression levels of TRF2 mRNA of follicular lymphoma, mantle cell lymphoma and diffuse large B cell lymphoma were(22 943±9 424)amol, (23 181±5 983)amol and (18 339±7 910)amol, respectively, which had no significant difference compared with reactive lymphoadenopathy [(21 796±4 800)amol, P >0 05]. The expression level of TRF2 mRNA of Burkitt lymphoma was (33 170±12 841)amol, which was significantly higher than that of reactive lymphoadenopathy and other types of NHL ( P <0 05). Conclusion: In NHL, expression of TRF2 mRNA in Burkitt lymphoma is significantly higher than that of follicular lymphoma, mantle cell lymphoma and diffuse large B cell lymphoma.
作者 钱文斌 孟海涛 金洁
机构地区 浙江大学医学院附属第一医院
出处 《浙江大学学报（医学版）》 CAS CSCD 2004年第5期416-420,共5页 Journal of Zhejiang University(Medical Sciences)
基金 浙江省自然科学基金 ( 30 0 5 0 7 M30 3773) 浙江大学医学院中青年科技启动基金资助项目
关键词 实时RT—PCR/方法 淋巴瘤 非霍奇金 端粒结合因子2 Real-time RT-PCR/method Lymphoma, non-Hodgkin Telomere binding factor 2
分类号 R733.1 [医药卫生—肿瘤]
  • 相关文献

参考文献13

  • 1ARTANDI S E, ALSON S, ITITZE M K, et al. Constitutive telomerase expression promotes mammary carcinomas in aging mice [J]. Proc Natl Acad Sci USA, 2002,99(12):8 191-8 196.
  • 2OHYASHIKI J H, SAHIDA G, TAUCHI T, et al. Telomeres and telomerase in hematologic neoplasia [J]. Oncogene,2002,21(4):680-687.
  • 3SMOGORZEWSKA A,VAN STEENSEL B,BIANCHI A,et al.Control of human telomere length by TRF1 and TRF2 [J]. Mol Cell Biol, 2000,20(5):1 659-1 668.
  • 4VAN STEENSEL B,SMOGORZEWSKA A,DE LANGE T. TRF2 protects human telomeres from end-to-end fusion [J]. Cell, 1998,92(3):401-413.
  • 5KARLSEDER J, SMOGORZEWSKA A, DE LANGE T. Senescence induced by altered telomere state, not telomere loss [J]. Science, 2002,295(5 564):2 446-2 449.
  • 6KARLSEDER J, BROCCOLI D, DAI J, et al. p 53 and ATM-dependent apoptosis induced by telomeres lacking TRF2 [J]. Science, 1999,283(5406):1 321-1 325.
  • 7JAFFE E S, HARRI N L, STEIN H, et al. Pathology and genetic of tumours of haematopoietic and lymphoid tissues [M].New York:IARC Press,2001.
  • 8CHEUNG I Y, CHEUNG N. Quantitation of marrow disease in neuroblastoma by real-time reverse transcription-PCR [J]. Clin Cancer Res, 2001,7(6):1 698-1 705.
  • 9WATTJES M P, KRAUTER J, NAGEL S, et al. Comparison of nested competitive RT-PCR and real-time RT-PCR for the detection and qualification of AML/MTG8 fusion transcripts in t(8;21) positive acute myelogenous leukemia [J]. Leukemia, 2000,14(2):329-335.
  • 10BIECHE I, NOGUES C, PARADIS V, et al. Quantitation of hTERT gene expression in sporadic breast tumors with a real-time reverse transcription-polymerase chain reaction assay [J]. Clin Cancer Res, 2000,6(2):452-459.

二级参考文献4

  • 1金冬雁（译），分子克隆实验指南（第2版），1992年，19-22,42-49,259-262页
  • 2颜子颖（译），精编分子生物学实验指南，1998年，628页
  • 3Broccoli D，Nat Genet，1997年，17卷，671期，231页
  • 4Chong L，Science，1995年，270期，5242,1663页

共引文献3

同被引文献30

  • 1林进,谢珏,钱文斌.系统性红斑狼疮患者外周血CD4^+和CD8^+细胞端粒长度及端粒酶活性的研究[J].浙江大学学报（医学版）,2005,34(6):534-537. 被引量：3
  • 2陈斌,何超,劳伟峰,黄学锋,方炳良.端粒酶启动子肿瘤靶向TNF相关凋亡诱导配体基因抗大肠癌细胞HT-29的活性研究[J].浙江大学学报（医学版）,2006,35(1):45-49. 被引量：2
  • 3童向民,金洁,姚航平,钱文斌,孟海涛.人端粒酶逆转录酶和人白细胞介素18融合基因真核表达载体的构建和功能研究[J].浙江大学学报（医学版）,2006,35(4):360-365. 被引量：1
  • 4Holst D,Grimaldi PA.New factors in the regulation of adipose differentiation and metabolism[ J ].Curr Opin Lipidol,2002,13(4):241 -5.
  • 5YONG-MING LIU,JEAN-MARC LACORTE,et al.Adiponectin gene expression in subcutaneous adipose tissue of obese women in response to short-term very low calorie diet and refeeding[ J ].J Clin Endocrinol Metab,2003,88(12):5881 -6.
  • 6E Schoof,A Stuppy,F Harig,et al.Comparison of leptin gene expression in different adipose tissues in children and adults[ J ].Eur J Endorinol,2004,150 (9):579-84.
  • 7Seufert J,Kieffer TJ,Leech CA,et al.Leptin suppression of insulin secretion and gene expression in human pancreatic islets:Imp lications for the development of adipogenic diabetes mellitus[ J ].J Clin Endocrino 1M etab,1999,84 (3):670.
  • 8Joseph A.Sennello,Raja Fayad,et al.Regulation of T Cell-Mediated hepatic inflammation by adiponectin and leptin[ J ].Endocrinology,2005,146,(5):2157-64.
  • 9Rahmouni K,Haynes WG,Mark AL,et al.Cardiovascular and sympathetic effect of leptin[ J ].Curr Hypertens Rep,2002,4(2):119 -25.
  • 10Jing-Ning Huan,Ji Li,Yiping Hah,et al.Adipocyte-selective reduction of the leptin receptors induced by antisense leads to increased adiposity,dyslipidemia,and insulin resistance[ J ].J Biol Chem,2003,278 (46):45638 -50.

引证文献2

二级引证文献5

浙江大学学报（医学版）
2004年 第5期

相关作者

内容加载中请稍等...

相关机构

内容加载中请稍等...

相关主题

内容加载中请稍等...

浏览历史

内容加载中请稍等...
;
使用帮助 返回顶部