摘要
目的 :观察肺心病患者血清对兔肺动脉内皮细胞(RPAECs)DNA合成、损伤、一氧化氮(NO)合成、内皮素(ET)分泌的影响。方法 :用胶原酶I消化分离获得兔肺动脉内皮细胞 ,在含不同浓度肺心病患者或正常人血清的培养基孵育12h ,应用MTT比色法检测细胞增殖情况 ;应用单细胞电泳法检测细胞DNA的损伤 ;通过检测细胞培养上清中乳酸脱氢酶 (LDH)、ET、NO等指标观察RPAECs分泌功能的变化。结果 :高浓度肺心病患者血清对RPAECs细胞增殖有明显抑制作用 ,其中部分细胞脱落死亡 ;肺心病患者血清浓度为10 %、15 %、20%时LDH释放量显著高于正常对照组 ;随着肺心病患者血清浓度的升高 ,NO较正常对照合成量下降 ,而ET分泌量显著升高 ,且两者有显著相关性。结论 :高浓度肺心病患者血清对RPAECs有毒性作用 ,损伤DNA ,且抑制DNA合成。
Objective:To investigate the change of DNA synthesis,LDH,NO,or ET secreˉtion by rabbit pulmonary artery endothelial cells(RPAECs)when treated with different conˉcentrations'cor pulmonale patients'sera compared with that treated with normal donors'sera in order to further explore the etiology of cor pulmonale.Methods:The cells were dispersed by collagenase I according to Jaffe's method with modification.RPAECs of the forth to sixth generation were employed in the experiment.The cells were incubated with2.5%,5%,10%,15%,or20%sera from cor pulmonale patients or normal donors respectivelyr for about12h.Extent of proliferation was assayed by MTT chromatometry.Comet electrophoresis was used to evaluate the extent of DNA damage.LDH,NO,or ET concentration was assayed in the suˉpernatant.Results:Proliferation in the group treated with patients'sera in higher concentration was much lower than that of the normal group.Some cells even died.Release of LDH was inˉcreased significantly(in10%、15%、20%patients'sera treated groups)compared with respecˉtive normal control.It was found that the higher the concentration of patients'sera,the lower the secretion of NO,while,the higher the sera's concentration,the higher the secretion of ET.The production of ET was correlated with that of NO.Conclusion:It is suggest that high concentration sera of cor pulmonale patients do harm to RPAECs,resulting in DNA damage,and synthesis inhibition.
出处
《天津医科大学学报》
2004年第3期352-355,共4页
Journal of Tianjin Medical University
基金
天津市卫生局科研基金资助
(项目编号039-300006)
