摘要
用^(60)Coγ射线辐照2个杂交组合高世代材料中分离出的黄籽和黑籽甘蓝型油菜(Brassica napus L.)近等基因系种子,测定辐照前、后种子在发芽过程中的种子活力,超氧物歧化酶(SOD)和脂质过氧化产物丙二醛(MDA)的变化。结果表明,辐照后,黄籽比黑籽的SOD活性上升幅度小,MDA含量上升幅度大,种子活力下降较多。黄籽油菜种子的辐射敏感性比黑籽强;油菜的辐射抗性或敏感性不仅与体内保护酶水平有关,也可能与种皮的特殊保护结构和保护物质有关。
Seeds of a set of near-isogenic lines (Brassica napus L.) with different seedcoat color from yellow to black were irradiated by 60Coγ-rays of 150 krad.Seed vigor,superoxide dis-mutase (SOD) and malondialdehyde (MDA) in germinating seeds were analysed.In these characters,no significant difference between yellow-seeded lines (YLs) and black-seeded lines (BLs) showed before irradiation.But after irradiation,SOD activity in YLs was lower than that in BLs.While MDA content in YLs was obviously higher than that in DLs.As a result of irradiation,seed vigor of YLs was lower than that in BLs.These results indicated that the irradiation resistance of rapeseed was related to the level of SOD as well as protective structure or substances in seedcoat and that the radiosensitivity of YLs was higher than that of DLs.
出处
《核农学报》
CAS
CSCD
1993年第3期139-144,共6页
Journal of Nuclear Agricultural Sciences
关键词
油菜
种子
超氧物歧化酶
辐照
Yellow-seeded rapeseed,60Coγ-rays, SOD, MDA, seed vigor
