摘要
利用血清学、RT-PCR并结合核苷酸序列测定等方法 ,对河南省地黄病毒病进行了初步鉴定。结果表明 ,烟草花叶病毒 ( TMV)为侵染地黄的主要病毒 ;对 TMV地黄分离物 ( TMV-RH) CP基因的序列分析结果表明 ,TMV-RH与 TMV-U1株系 CP基因的核苷酸同源性为 86.5 % ,氨基酸同源性为 94.3 % ;与已发表的 TMV其它株系 CP基因的核苷酸同源性在76.3 %～ 88.5 %之间 ,氨基酸同源性在 79.3 %～ 95 .0 %之间 ,同源性较低。根据不同株系 CP的氨基酸序列进化树分析 ,推测该分离物可能为
Virus species infected Rehmannia glutinosa were detected by using ELISA, RT PCR and nucleotide (nt) sequence analysis of coat protein (CP) gene. The results revealed that Tobacco mosaic virus (TMV) was the major virus infecting Rehmannia glutinosa in Henan Province. CP gene of TMV from Rehmannia glutinosa (TMV RH) was cloned and sequenced. The sequence analysis showed that the nt and amino acid (aa) sequence of TMV RH CP were 86.5% and 94.3% identical to TMV U1 strain, and were 76.3% to 88.5% and 79.3% to 95.0% identical to other TMV strains, respectively. According to the results of phylogenetic tree generated based on aa sequences of CP genes, it is supposed that TMV RH may be a new strain of TMV.
出处
《植物病理学报》
CAS
CSCD
北大核心
2004年第5期395-399,共5页
Acta Phytopathologica Sinica
基金
河南省杰出人才创新基金 ( 0 2 2 1 0 0 0 70 0 )
国家攻关引导项目 ( 2 0 0 3BA76 1 C)
关键词
地黄
病毒病
烟草花叶病毒
株系
Rehmannia glutinosa
virus disease
Tobacco mosaic virus
strain
