青光眼患者房水中肝细胞生长因子的临床研究被引量：3

Clinical investigations on the hepatocyte growth factor in aqueous humor of glaucomatous eyes

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摘要目的 :检测不同类型青光眼患者房水中的肝细胞生长因子 (hepatocytegrowthfactor,HGF)浓度并与单纯白内障患者作比较 ,初步探讨HGF在青光眼发病中的作用。方法 :取不同类型青光眼及单纯白内障患者的房水样本 0 1～ 0 2ml,采用双抗体夹心法酶联免疫吸附试验 (enzyme linkedimmunosorbentassay ,ELISA)测定其HGF浓度。结果 :2 0例原发性急性闭角型青光眼组房水HGF为 (32 19 74 5 1± 32 99 2 4 4 1) pg/ml;2 8例原发性慢性闭角型青光眼组HGF为 (86 2 4 2 86± 2 5 5 885 1)pg/ml;18例原发性开角型青光眼组HGF为 (10 72 5 72 3± 5 0 4 885 0 ) pg/ml;2 8例单纯白内障对照组HGF为 (6 85 314 3±173 7970 )pg/ml。三组青光眼与白内障对照组比较均有显著性差异 (P <0 0 5 ) ,并且急性闭角型青光眼组与慢性闭角型青光眼组和开角型青光眼组比较具有非常显著性差异 (P <0 0 1) ,慢性闭角型青光眼组与开角型青光眼组比较 (P =0 815 )没有显著性差异。结论 :HGF可能与青光眼发病后的组织损伤有关。 Objective:To assess the concentration of hepatocyte growth factor (HGF) in the aqueous humor of glaucoma eyes,comparing with those of eyes with cataract only,used as control,in order to assess the role of HGF in the pathophysiology of glaucoma.Methods: 0 1-0 2ml of aqueous humor were collected from 76 primary glaucoma eyes and 28 eyes with simple cataract,which were used as control.The concentration of HGF were measured with double antibody Sandwich engyme linked immunosorbent assay (ELISA).In view of the normal distribution with 1 Sample K S Test,the HGF concentrations in patient groups were compared,and the statistical significance of the results was analyzed with One Way ANOVA.Results:The concentration of HGF in eyes with simple cataract (n=28) was 685 3143±173 7970 pg/ml (mean±standard deviation),which was significantly lower ( P <0 05) than that in eyes with primary acute angle closure glaucoma (n=20,3219 7451±3299 2441 pg/ml) or primary chronic angle closure glaucoma (n=28,862 4286±255 8851 pg/ml) or primary open angle glaucoma (n=28,1072 5723±504 8850 pg/ml). In addition,eyes with acute angle closure glaucoma had significantly higher HGF concentrations than eyes with chronic angle closure glaucoma or primary open angle glaucoma ( P <0 01),whereas no significant difference was found between groups of chronic angle closure glaucoma and open angle glaucoma ( P =0 815). Conclusion:The higher concentration of HGF in glaucoma patients aqueous humor may indicate a response to injury after glaucoma.

作者王书华刘磊王宁利张舒心林丁

机构地区首都医科大学附属北京同仁医院眼科中心

出处《眼科》 CAS 2004年第4期207-210,共4页 Ophthalmology in China

关键词青光眼肝细胞生长因子酶联免疫吸附试验 glaucoma hepatocyte growth factor (HGF) enzyme linked immunosorbent assay (ELISA)

分类号 R775 [医药卫生—眼科]

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