摘要
目的 利用单克隆抗体代替常规的人、猴免疫血清进行甲型肝炎减毒活疫苗毒种外源因子检查及鉴别试验。方法 选择适宜的单克隆抗体浓度与甲肝病毒进行中和试验 ,观察单克隆抗体的中和作用 ;利用单克隆抗体中和毒种 ,进行外源因子检查 ,并应用于鉴别试验。结果 此单克隆抗体可中和 10 5细胞培养半数感染量 (CCID50 )甲肝病毒 ,比人、猴免疫血清的中和能力高约 10 0倍 ;将此高效价的单克隆抗体中和毒种后进行血吸附及非血吸附外源因子检查 ,结果均为阴性 ;此单克隆抗体还可用于毒种鉴别试验。结论 此高效价的单克隆抗体可代替人、猴免疫血清应用于毒种的外源因子检查及鉴别试验。
Objective To identify live attenuated hepatitis A(HAV) vaccine virus seed and detect adventitious agents,monoclonal antibody is used to neutralize instead of human and rhesus monkey immune serum.Methods Choosing suitable monoclonal antibody concentration to neutralize HAV for confirming the neutralization capacity of monoclonal antibody.To test adventitious agents after monoclonal antibody neutralized HAV vaccine virus seed,and further apply to identification test.Results The monoclonal antibody(1∶100) could neutralize 10 5 CCID 50 /ml HAV virus and it was 100 times than that of human or rhesus monkey immune serum;Applied that high efficiency monoclonal antibody to hemadsorption test and non-hemadsorption test for adventitious agents,the results were negative and the monoclonal antibody could be used in identification test.Conclusion With high-efficiency monoclonal antibody on adventitious agents detection,it can be applied a small scale dilution of HAV virus seed to aviod the difficult of testing adventitious agents by large scale dilution of vaccine virus seed.The monoclonal antibody was used to neutralize instead of human and rhesus monkey immune serum.
出处
《中国公共卫生》
CAS
CSCD
北大核心
2004年第7期808-809,共2页
Chinese Journal of Public Health
关键词
单克隆抗体
甲型肝炎
减毒活疫苗毒种
外源因子
鉴别试验
monoclonal antibody
HAV
live attenuated vaccine virus seed,adventitious agent
identification test
