摘要
目的研究犬单侧膈神经离断后不同时期呼吸肌腺嘌呤核苷酸含量变化。方法选取8只成年健康杂种犬,离断其左侧膈神经,分别取其同侧肋间肌和膈肌组织,用高效液相色谱法测量膈神经离断前及离断后1、2个月呼吸肌中腺嘌呤核苷酸含量。结果犬肋间肌三磷酸腺苷(ATP)的含量三个时间组相比均无差别;而二磷酸腺苷(ADP)的含量上升,膈神经离断后2月组与离断后1月组相比,有统计学差别(P<0.05);犬肋间肌总腺苷酸(TAN)含量以膈神经离断后1月组为最低,但并无统计学差别;肋间肌腺苷酸能荷(AEC)以膈神经离断后1个月为最低,与离断前组和离断后2月组相比,均有统计学差别(P<0.05)。犬的膈肌ATP、ADP及TAN、AEC含量在膈神经离断后2个月分别为9.05±12.70,2.99±2.57,14.72±13.98,0.57±0.29;与膈神经离断前及离断后1个月相比,有统计学意义(P<0.05)。结论犬膈神经离断后膈肌能量代谢明显下降,肋间肌有一定程度代偿。
Objective To examine the changes in energy metabolism in the respiratory muscles of canines with unilateral phrenic nerve transection using high-performance liquid chromatography (HPLC). Methods The left phrenic nerve of 8 canines was transected and the contents of adenine nucleotide in respiratory muscles were determined by HPLC before and 1 and 2 months after the operation. Results In the intercostal muscles, ATP contents remained almost unchanged during the entire course of the observation, while ADP content was elevated 2 months after the operation as compared with that measured before and 1 month after the operation (P<0.05). One month postoperatively, the content of total adenylic acid (TAN) decreased to the lowest point, but the difference between the measurements was not statistically significant; also at 1 month after the operation, the content of adenylate energy charge (AEC) was the lowest, with statistically significant difference from the measurements before and 2 months after the operation (P<0.05). In the diaphragm, the contents of ATP, ADP, TAN and AEC 2 months postoperatively were 9.05γ from the levels at the other two time points (P<0.05). Conclusion After unilateral phrenic nerve transection in canines, the energy metabolism of the diaphragm declines significantly, whereas that of the intercostal muscle can be compensated to some extent.
出处
《第一军医大学学报》
CSCD
北大核心
2004年第8期922-925,共4页
Journal of First Military Medical University
关键词
膈神经切断术
呼吸肌
能量代谢
高效液相色谱法
phrenicectomy
respiratory muscles
energy metabolism
high-performance liquid chromatograpy
