摘要
目的 :探讨牛磺酸对失血性休克复苏后血浆和心肌一氧化氮合酶 (NOS)活性、一氧化氮 (NO)含量变化的影响。方法 :新西兰种兔 2 4只随机分为 3组 (n =8) :对照组、休克组、牛磺酸治疗组。采用失血性休克复苏动物模型。连续观察休克前、休克 1.5h、复苏后 1h、2h、3h血浆一氧化氮合酶 (NOS)活性、一氧化氮代谢产物 (NO-2 /NO-3 )含量、乳酸脱氢酶 (LDH)活性的动态变化。测定复苏后 3h心肌一氧化氮合酶 (NOS)活性、一氧化氮代谢产物 (NO-2 /NO-3 )含量的变化 ,并常规留取心肌标本观察形态学改变。结果 :①休克组复苏后各时限血浆NOS活性、NO-2 /NO-3 含量、LDH活性显著高于休克前及休克 1.5h ;②休克组复苏后 3h心肌NOS活性、NO-2 /NO-3 含量显著高于对照组 ,心肌出现明显水肿和脂肪变性 ;③牛磺酸 (4 0mg·kg-1iv)可显著缓解上述变化。结论 :失血性休克复苏后NOS的激活和NO的大量释放 ,可能介导了休克复苏所致心肌损伤 。
Aim: To investigate the effect of taurine on nitric oxide synthase(NOS) activity and nitric oxide products(NO^-_2/NO^-_3) content in myocardium and plasma during shock resuscitation. Methods: Twenty-four rabbits were divided randomly into 3 groups(n=8): control group, shock group, taurine group. The model of hemorrhagic shock resuscitation was used. The activities of nitric oxide synthase(NOS), lactate dehydrogenase(LDH) and the contents of nitric oxide products(NO^-_2/NO^-_3) in plasma were observed before shock and shock 1.5 hours,after resuscitation 1 hour,2 hours and 3 hours. The activities of NOS and the contents of NO^-_2/NO^-_3 in myocardium homogenate were measured after resuscitation 3 hours. Meanwhile,pathologic samples treated routinely. Results:①During resuscitation, the activities of NOS,LDH and the contents of NO^-_2/NO^-_3 in plasma of shock group were significantly higher than that of before shock and shock 1.5 hours(P<0.01). ②Atfter resuscitation 3 hours, the activity of NOS and the contents of NO^-_2/NO^-_3 in myocardium of shock group were significantly higher than that of control group(P<0.01).The cardiac myocyte appeared edema, fattydegeneration. ③All the changes of above mentioned could be attenuated by intravenous injection taurine(40 mg/kg)(P<0.01).Conclusion: These results suggest that the NOS activation and NO release may mediated myocardium injury induced by shock resuscitation, taurine can ameliorate the myocardium injury, which may be related to decreasing the generation of NO.
出处
《中国应用生理学杂志》
CAS
CSCD
北大核心
2004年第3期235-237,F005,共4页
Chinese Journal of Applied Physiology
关键词
休克
复苏
一氧化氮
牛磺酸
shock
resuscitation
nitric oxide
taurine
myocardium
