抗CCP等4种自身抗体联合检测在RA诊断中的应用被引量：3

Application in the diagnosis of rheumatoid arthritis combinedmeasuring including anti-cyclic citrullinase peptide antibodyand other three auto-antibodies

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摘要目的　探讨抗环瓜氨酸肽 (CCP)抗体 ,抗角蛋白抗体 (AKA)及相关自身抗体在类风湿关节炎 (RA)诊断中的意义。方法采用法ELISA检测抗CCP抗体 ,间接免疫荧光法检测AKA、ANA(抗核抗体 ) ;类风湿因子 (RF)用免疫比浊法测定。共检测 134例 ,其中类风湿关节炎 (RA)患者 6 2例、系统性红斑狼疮 (SLE)患者 2 5例、骨关节炎 (OA)患者 9例、强直性脊柱炎 (AS)患者 8例和正常对照 30例。结果 6 2例RA患者中抗CCP、AKA、ANA和RF的阳性率分别是 4 8.4 % (30 / 6 2 )、38.7% (2 4 / 6 2 )、5 3.2 %(33/ 6 2 )和 5 9.7% (37/ 6 2 ) ;2 5例SLE患者的阳性率是 4 .0 % (1/ 2 5 )、4 .0 % (1/ 2 5 )、88.0 % (2 2 / 2 5 )和 32 .0 % (8/ 2 5 ) ;9例OA患者无一例阳性 ;8例AS中ANA阳性率为 12 .5 % (1/ 8) ;30例正常对照中有一例RF阳性 ,其阳性率为 3.3% (1/ 30 )。结论抗CCP抗体和AKA抗体为RA特异性诊断指标 ,联合检测有助于提高临床对RA的早期诊断率。 ObjectiveTo study the significance of including anti-cyclic citmllinase peptide antibody(anti-CCP), AKA and other related auto-antibodies in diagnosing rheumatoid arthritis(RA). Methods Anti-CCP was measured by ELISA, AKA and ANA were detected by indirect immunofluorescence assay, while RF was analyzed by immuno-turbidimetric method.Among 134 patients, there were 62 cases with RA, 25 cases with SLE, OA 9 cases, AS 8 cases and 30 normal controls. Results The positive ratios of anti-CCP, AKA, ANA and RF in the 62 patients with RA were 48.4%O(30/62), 38.7%(24/62), 53.2%(33/62) and 59.7%O(37/62), respectively, while in the 25 cases with SLE were 4.0%(1/25), 4.0%(1/25), 88.0%(22/25) and 32.0%(8/25), respectively. The 90A patients were not found any of the four autoantibodies was positive, and 12.5%(1/8) positive ratio of ANA in the 8 AS cases, only one patient with positive RF among the 30 controls(3.3%, 1/30). Conclusion combined detection of anti-CCP and AKA may provide a specific and early index in diagnosing RA.

作者何兰唐中凡瞿明张国元

机构地区川北医学院附属医院检验科

出处《川北医学院学报》 CAS 2004年第2期90-92,共3页 Journal of North Sichuan Medical College

关键词抗CCP 自身抗体联合检测 RA 诊断类风湿关节炎 anti-cyclic citrullinase peptide antibody AKA rheumatoid arthritis

分类号 R593.22 [医药卫生—内科学]

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