摘要
目的 了解褪黑素 (MT)对衰老小鼠肺炎时心肌的保护作用。方法 将雄性NIH小鼠 10 0只随机分为两组 :一组以D 半乳糖制作人工老化模型 (D gal组 ) ,另一组以生理盐水作对照 (NS组 )。每组分别分为 4个亚组 (12只 )进行实验 :生理盐水对照组 (NSN ,D galN)、肺部感染组 (NSP ,D galP)、褪黑素 +肺部感染组 (NSM ,D galM)、溶媒 +肺部感染组(NSV ,D galV)。制模后 72h观察心肌线粒体形态改变 ,检测血浆中TNF α、心肌组织中丙二醛 (MDA)、TNF α、ATP含量、总超氧化物歧化酶 (T SOD)和铜、锌超氧化物歧化酶 (Cu ,Zn SOD)活性。结果 肺部感染后小鼠心肌组织中MDA、TNF a含量增加 (NSNvsNSP ,D galNvsD galP ;P <0 .0 5 )T SOD和Cu ,Zn SOD活性、ATP含量降低 (P <0 .0 5 ) ,心肌组织线粒体损伤较多。MT干预可部分抑制上述改变、减少线粒体变性 (NSMvsNSP ,D galMvsD galP ;P <0 .0 5 )。结论 MT通过改善心脏抗氧化功能、降低心肌脂质过氧化、降低血浆和心肌炎性因子含量等作用保护肺炎对人工老化小鼠心脏功能的损害。
Objective To study the influence of melatonin (MT) on the heart of the artificially accelerated aging mouse with pneumonia. Methods One hundred male NIH mice were randomly divided into 2 groups and were pretreated with D-galactose(D-gal) or normal saline(NS) respectively. Then, each group was divided into 4 subgroups: the normal group (injected with normal saline directly through trachea)(N), pneumonia group (Pseudomonas aeruginosa saline suspension was injected directly through trachea to induce pneumonia)(P), MT+pneumonia group and vehicle+pneumonia group (MT or vehicle was given once a day by intraperitoneal injection throughout the induced pneumonia experiment). Results The value of plasma TNF-α(NSN vs NSP, D-galN vs D-galP, P<0.05), and the contents of TNF-α and MDA(P<0.05) in cardiac tissue were increased, whereas the content of ATP, the activities of T-SOD and Cu, Zn-SOD(P<0.05) were reduced in mice of pneumonia or vehicle+pneumonia groups. The damage of myocardial mitochondria was prevalent in pneumonia groups. The changes of above parameters and myocardial mitochondria in MT+pneumonia group were partially reduced(P<0.05). Conclusion MT may partially reduce the myocardial lipid peroxidation and myocardial mitochondria impairment, and recover the ability of energy metabolism after occurrence of pneumonia in aging mouce.
出处
《中华老年多器官疾病杂志》
2004年第2期116-119,共4页
Chinese Journal of Multiple Organ Diseases in the Elderly
基金
国家重点基础研究发展规划基金资助 (编号G2 0 0 0 0 5 70 0 4)
