摘要
目的:探讨国产巴西蘑菇多糖对造血干、祖细胞生成的影响及其作用机制。方法:应用造血细胞培养技术、流式细胞仪(FACS)、逆转录多聚酶链反应(RT-PCR)观察药物对小鼠骨髓细胞及人脐血单个核细胞的影响。结果:经过巴西蘑菇多糖处理的动物,造血干细胞(CFU-S)、粒-单祖细胞(CFU-GM)、红系祖细胞(CFU-E)和纤维母细胞(CFU-F)各组数值明显上升,与对照组比较,P均<0.01;FACS检测显示,实验组CD33+细胞明显上升,在诱导的d 7达高峰;经RT-PCR检测结果表明,巴西蘑菇多糖诱导人脐血单个核细胞48 h后,粒单细胞克隆刺激因子(GM-CSF)mRNA的表达量是对照组的2.72倍。结论:巴西蘑菇多糖可促进造血干、祖细胞生成并可促进人脐血单个核细胞GM-CSF基因表达。
AIM: To explore the effect of Agaricus Blazei polysaccharide (ABPS) on the hematopoietic stem celland progenitor cells. METHODS: Bone marrow nuclear cells from mouse were used to the culture of hematopoietic stem cell in vivo and progenitor cells in vitro. The cord blood cells were used to reverse transcription-polymerase chain reac tion(RT-PCR) for granulocyte-macrophage colony stimulating factor(GM-CSF) messenger RNA(mRNA) assay. RESULTS: ABPS could promoting colony forming unit-spleen(CFU-S), colony forming unit-granulocyte-macrophage (CFU-GM),colony forming unit-erythrcyte (CFU-E) and colony forming unitt-fibroblasts(CFU-F) growing and increased GM-CSF mRNA expression in the cord blood,and significant differences were found as compared with the control groups( P < 0.01). In another experiment, the number of CD33 + cells was increased by ABPS inducing for 7 days. CONCLUSION: ABPS could be used for promoting the growth of hematopoietic stem and progenitor cells, and increasing the expression of GM-CSF mRNA in human mononuclear cells from cord blood.
出处
《中国临床药学杂志》
CAS
2004年第4期204-207,共4页
Chinese Journal of Clinical Pharmacy
基金
上海市高等学校科学技术发展基金(02DK09)甘肃省科委重点基金(GS981A42149)
关键词
巴西蘑菇多糖
造血干细胞
造血祖细胞
细胞生成
粒单细胞克隆刺激因子
Agaricus Blazei polysaccharide
hematopoetic stem cell
hematopoietic progenitor cells
upregulation
GM-CSF mRNA expression
