摘要
目的探讨复方贯叶金丝桃颗粒的药理作用。方法采用体外培养2.2.15细胞系感染乙肝病毒模型,鸭感染乙肝动物模型,用CCl4、D-氨基半乳糖胺(D-GL)、BCG+LPS3种肝损伤小鼠模型,用固相放射免疫测定、斑点杂交放射自显影检测、MTT等测定法,观察其对乙肝病毒复制、对动物模型肝脏保护作用及对免疫功能的影响。结果在2.2.15细胞培养中,125μg/mL对HBsAg、HBeAg和HBV-DNA的平均抑制率分别为(29.8±2.3)%,(36.5±4.7)%和(30.3±8.8)%;在雏鸭乙肝模型中,4.0、6.0、8.0g/kg,均可抑制血清DHBV-DNA复制,尤以8.0g/kg作用更显著,作用较拉米夫定弱,但持效时间比其长。在CCl4、D-GL,BCG+LPS三种肝损伤模型中,1.25,2.50,5.00g/kg,有降低AST、ALT和保护肝细胞的作用,体内可提高小鼠HC50量,体外在0.01～10mg/L范围内可提高T淋巴细胞增殖率。结论复方贯叶金丝桃颗粒有减少HBsAg、HBsAg分泌,抑制DHBV-DNA复制,保肝降酶和免疫调节作用。
Objective To investigate the pharmacological actions of Compound Hypericum Perforutum granule(CHPG). Methods Duck models with hepatitis B induced by 2.2.15 cell strain were used to observe the effect of CHPG on the replication of hepatitis B virus(HBV).Mice models of liver injury induced by CCl4,D-aminogalactose and BCG+LPS were applied to observe the protective effect of CHPG on liver and mice immune function.Solid-phase radioimmuoassay, spot-hybridism radiation self-developing method and MTT method were adopted.Results The average inhibitory rate of CHPG 125 μg/mL was(29.8±2.3)%,(36.5±4.7)%and(30.3±8.8)%for HBsAg,HBeAg and HBV-DNA respectively.In the duckling hepatitis B model,CHPG 4.0,6.0,8.0 g/kg had an obvious effect in inhibiting the replication of DHBV-DNA,CHPG 8.0 g/kg in particular;the effect was weaker but maintained longer time than lamivudine.1.25,2.50,5.00 g/kg of CHPG could decreased serum levels of AST and ALT and protect liver cells mice with liver injury and increased serum hemolysin concentration(HC50).0.01～10 mg/L of CHPG accelerated the proliferation of T lymphocyte of mice in vitro.Conclusion CHPG is effective in reducing the secretion of HBsAg,HBeAg in vitro,inhibiting the replication of DHBV-DNA in duck hepatitis B model.It has effect in protecting liver cells,decreasing ALT and AST levels and regulating immune function.
出处
《中药新药与临床药理》
CAS
CSCD
2004年第4期238-241,共4页
Traditional Chinese Drug Research and Clinical Pharmacology
基金
国家中医药管理局资助项目(编号DIX032A)
新疆维吾尔自治区科技厅重点课题(编号20000103001)
关键词
复方贯叶金丝桃颗粒
乙型病毒性肝炎
保肝降酶
免疫调节
Compound hyperieum perforatum granule
Virus hepatitis B
Protecting liver and decreasing enzyme
Immunoregulation
