摘要
目的探讨解毒化瘀汤对D-氨基半乳糖胺(D-GalN)所致小鼠肝损伤的保护作用。方法NIH小鼠60只,随机分5组,分别为正常对照组,模型组,解毒化瘀汤高剂量组、低剂量组和齐墩果酸片治疗组;解毒化瘀汤高、低剂量组和齐墩果酸片组小鼠分别灌胃给药,1次/d,连续4 d,末次给药1 h后,除正常对照组外,其余各组小鼠均腹腔注射D-GalN 800 mg/Kg,24 h后采血,检测血清ALT活性、超氧化物歧化酶(SOD)活性和丙二醛(MDA)含量,并观察肝组织病理学变化。结果解毒化瘀汤高剂量组血清ALT活性和MDA含量均明显低于模型组(P<0.01)、小鼠血清SOD的活性明显高于模型组(P<0.01);与齐墩果酸片组比较,解毒化瘀汤高剂量组ALT,SOD活性和MDA含量差异均无显著性意义(P>0.05);解毒化瘀汤高剂量组肝组织病理改变明显减轻,与模型组比较差异均有显著性意义(P<0.05)。结论解毒化瘀汤对D-GalN所致小鼠肝损伤具有较好的保护作用,其保肝机制与抗脂质过氧化损伤密切相关。
Objective To investigate the protective effects of Jieduhuayutang （JDHYT） on liver injury in mice induced by D- galactosamine （D-GaiN）. Methods Sixty NIH mice were randomly divided into 5 groups： normal control group（ group A）, model group （group B）, high dosage group of JDHYT （group C）, small dosage group of JDHYT （group D） and Oleanolic Acid Tablets group （group E）. Mice in group C, group D and group E were administered with drugs by gastric perfusion for 4 days once daily consecutively. One hour after the last administration of the drugs, excepts those in group A , mice in other groups were injected with D-GalN 800 mg/kg into its abdominal cavity. Blood samples for determining aminotransferase （ ALT）, superoxide dismutase（SOD） activity and malondiadehyde（MDA） concentration were collected at the 24h hour after D-GalN administration . Histopathological examination was also performed for liver tissue. Results Serum ALT and MDA in group C were obviously lower than that of group B （P 〈 0.01 ）, SOD obviously higher than that of group B （ P 〈 0.01 ）, but there was no significant difference compared with group E （ P 〉 0.05 ）. Hepatic histopathological changes were alleviated in group C compared with group B （ P 〈 0.05 ）. Conclusion JDHYT has a good protective effects on liver injury in mice induced by D-GalN, its therapeutic mechanism is related to the antioxiclation
出处
《时珍国医国药》
CAS
CSCD
北大核心
2006年第4期500-502,共3页
Lishizhen Medicine and Materia Medica Research
基金
广州中医药大学中药学院自然科学基金(No.0506)
