摘要
目的 探讨超声刀切割产生的烟雾中活性细胞的情况。方法 用超声刀对脾和胃组织进行不同时间 (5、10、15、2 0s)、功率的切割 ,收集切割时产生的烟雾进行贴壁细胞培养 2 4h后 ,用计数板计算培养液中活性细胞的绝对值。结果 超声刀功率为五档时 ,对脾组织不同时间切割产生的烟雾中活性细胞培养结果 (× 10 4个 ) :3 .675± 0 .194、3 6.0 75± 0 .5 61、75 .10 0± 0 .714、96.82 5± 0 .741。功率五档时 ,对胃组织进行不同时间切割产生的烟雾中活性细胞培养结果 (× 10 4个 ) :0 .0 0 0± 0 .0 0 0、0 .15 0± 0 .0 3 3、0 .65 0± 0 .0 62、0 .82 5± 0 .0 63。超声刀对脾组织切割产生的烟雾中活性细胞较多。对胃组织切割产生的烟雾中细胞数量极少。切割脾和胃组织时产生的烟雾中细胞数量差异有显著性。结论 超声刀切割脾和胃组织时 ,烟雾中都有一定量的活性细胞 ,活细胞数量与切割时间和功率成正比。烟雾中活性细胞数量与组织结构致密度有关。
Objective To study viable cells in smog produced by Ultrasonically Activated Scalpel(UAS) during tissue incision.To provide primary evidence of safe clinical using of UAS.Methods Incising tissue of spleen and stomach in different time(5?10?15?20 s) and power by UAS.Collecting their smog and culturing cells in smog,count viable cells 24h later.Results When incising spleen tissue in fifth grade and different time,the number of viable cells in smog are following (×10 4):3.675±0.194,36.075±0.561,75.100±0.714,96.825±0.741.When incising gastric tissue in fifth grade and different time,the number of viable cells in smog are following(×10 4):0.000±0.000,0.150±0.033,0.650±0.062,0.825±0.063.There are many viable cells in smog during incising spleen tissue but few viable cells in smog during incising gastric tissue.And there are difference of viable cells number between spleen incision and gastric tissues incision.Conclusion There are viable cells in smog during incising spleen and gastric tissues by UAS.Relationship between viable cells number and incision time and power is direct ratio.There are correlation between viable cell number and density of tissue structure.
出处
《中华实验外科杂志》
CAS
CSCD
北大核心
2004年第6期690-691,共2页
Chinese Journal of Experimental Surgery
关键词
超声刀
切割
烟雾
细胞
活性
胃组织
Ultrasonically Activated Scalpel
Viable Cells
Spleen
Stomach
