摘要
在血管及细胞膜通透性示踪的基础上,作者应用胶体金标记的抗肾综合征出血热病毒(HFRSV)多克隆和单克隆抗体,对陈株HFRSV感染的BALB/c乳鼠体内的抗原进行了体内示踪,光镜及电镜的观察.在对照动物中,示踪剂可清晰显示出组织内血管及网状内皮细胞系统,并出现于吞噬细胞的胞浆溶酶体中;在感染动物,胶体金标无关抗体示踪可显示出血管通透性的改变,但无明显组织定位特性;而金标HFRSV抗体可出现于各器官的实质细胞中伴有组织结构的改变,并定位于细胞胞浆内的游离核糖体、高尔基体、空泡内颗粒状结构及包涵体样结构.多数细胞结构破坏,并有异常结构的出现;但病毒颗粒、未成熟病毒颗粒及病毒包涵体只在少数细胞中观察到.结果说明,游离核糖体、高尔基体及细胞内泡状结构与病毒感染有关,病毒抗原阳性并非一定代表有完整的病毒结构.结果提示机体感染HFRSV后产生的抗体除可与细胞外抗原结合形成免疫复合物,造成组织的损伤外,还有透过损伤的细胞膜与细胞内抗原结合的可能性.
As the prior works done on the abnormal permeabilities of vasculatures and cellular membranes after hemorrhagic fever with renal syndrome virus (HFRSV) infection, the internalization of viral antigens in the experimentally infected suckling BALB/c mice with Chen strain HFRSV were further studied by application of the colloidal gold-labelled polyclonal and monoclonal antibodies against HFRSV (GLAb) to the infected animals via tail veins. The normal mice with GLAb and the infected mice with colloidal gold-labelled irrelated antibodies (GLIg) were also employed as experimental controls. At certain time intervals after the injection, the animals were sacrificed and the tissues were observed under light and electron microscopes. In the normal mice, the conjugates were only confined to the vasculatures and endo-reticulum system and occurred in the cytoplasmic lysosomes of phagocytes. In the infected animals, the GLIg conjugates could demonstrate the abnormal permeability, but could not show the antigen localization, while the GLAb could be internalized into the cytoplasms of the parenchymal cells and localized in the free ribonsome, Golgi apparatus, granules within vesicles, and inclusion bodies-like structures in the cytoplasms. Compared to the infected animals with GLIg, the obvious tissue structure alternation in LM was observed in the infected mice with GLAb. The ultrastructural changes or destruction and abnormal structures frequently occurred in the cells of infected animals. The typical virus, immature virons and inclusion body could be found but only in a few cells. The results indicated that the free ribosomes, Goigi apparatus and vesiculae may be related to the virus infection and the viral antigen positive could not represent the virus structure only, and suggested that the antibodies produced in the bodies after HFRSV infection could bind not only the extracellular viral antigens to form immunocomplexes to induce tissue lesions but also the cyloplasmic viral antigens of the infected cells through the damage cellular membranes.
出处
《第四军医大学学报》
1993年第4期251-256,共6页
Journal of the Fourth Military Medical University
基金
国家自然科学基金 No.39270290
关键词
肾综合征
出血热
病毒
乳鼠
感染
hemorrhagic fever with renal syndrome
virus
suckling mice
infection
experimental
antigens
microscopy
electron
