摘要
用光镜免疫组化和免疫电镜技术观察了8例正常人心肌中Cu-Zn SOD的亚细胞定位,并用放射免疫法测定了心肌中Cu-Zn SOD的含量。结果显示多数心肌细胞含有Cu-Zn SOD,心室肌Cu-Zn SOD含量略高于心房肌。Cu-Zn SOD金标记颗粒分布于胞浆及核中,线粒体中略少,溶酶体中仅有极少量分布,肌浆网和高尔基器等膜结构上几乎未见Cu-Zn SOD金标记颗粒。本研究的结果表明,心肌是Cu-Zn SOD含量较丰富的组织。
The distribution of copper-zinc superoxide dismutase (Cu-ZnSOD) in subcellular organelles in 8 cases of normal human myocardium was studied by using immunocytochemistry and immunoelectron microscopic technique and the contents of Cu-Zn SOD in myocardium were measured by radioimmunoassay. The results showed that Cu-ZnSOD was in most myocardial cells. The contents of Cu-ZnSOD in the ventricular myocardial cells were higher than those in the atrial myocardial cells. The Cu-ZnSOD labeled with immunogold particles were distributed throughout cytoplasm and nuclei, but less in mitochondria and a very little in lysosomes. No gold particle was found on the membranous structure of sarcoplasmic reticulum as well as Golgi complex. The results demonstrate that the myocardium was the tissue containing quite abundant Cu-Zn SOD.
出处
《第二军医大学学报》
CAS
CSCD
北大核心
1993年第3期229-232,共4页
Academic Journal of Second Military Medical University
关键词
心肌
超氧物歧化酶
免疫组织化学
myocardium
superoxide dismutases
immunohistochemistry
ultrastructure
radioimmunoassay
